{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Song C"],"funding":["National Key R&D Program of China","the Basic Agricultural Research Project of Wenzhou"],"pagination":["3781"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12472610"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["30(18)"],"pubmed_abstract":["Volatile organic compounds (VOCs) play critical roles in broccoli's sensory attributes, defense mechanisms, and ecological interactions, yet comprehensive profiling of its volatilome remains limited. This study aimed to construct a robust and inclusive volatile metabolite database for broccoli using advanced analytical techniques. A pooled sample comprising florets from 191 cultivars was prepared to capture broad chemical diversity and analyzed using solid-phase microextraction-gas chromatography-high-resolution mass spectrometry (SPME-GC-HRMS) under dual ionization modes: electron ionization (EI) and chemical ionization (CI). A total of 206 VOCs spanning nine chemical classes were detected, with 37 compounds further confirmed through synchronized CI analysis. To validate the database, bro"],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Dual-Ionization SPME-GC-HRMS Metabolomic Profiling of Broccoli Volatiles for the Construction of a Broccoli Metabolic Database."],"pmcid":["PMC12472610"],"funding_grant_id":["2022YFE0108300","N20220013"],"pubmed_authors":["Yan M","Zou H","Li J","Lin S","Hu Z","Song C","Yan X"],"additional_accession":[]},"is_claimable":false,"name":"Dual-Ionization SPME-GC-HRMS Metabolomic Profiling of Broccoli Volatiles for the Construction of a Broccoli Metabolic Database.","description":"Volatile organic compounds (VOCs) play critical roles in broccoli's sensory attributes, defense mechanisms, and ecological interactions, yet comprehensive profiling of its volatilome remains limited. This study aimed to construct a robust and inclusive volatile metabolite database for broccoli using advanced analytical techniques. A pooled sample comprising florets from 191 cultivars was prepared to capture broad chemical diversity and analyzed using solid-phase microextraction-gas chromatography-high-resolution mass spectrometry (SPME-GC-HRMS) under dual ionization modes: electron ionization (EI) and chemical ionization (CI). A total of 206 VOCs spanning nine chemical classes were detected, with 37 compounds further confirmed through synchronized CI analysis. To validate the database, bro","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-01T03:20:13.547Z","creation":"2026-05-01T03:11:20.391Z"},"accession":"S-EPMC12472610","cross_references":{"pubmed":["41011672"],"doi":["10.3390/molecules30183781"]}}