{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tao Y"],"funding":["Key Project of Regulatory Science Research of Anhui Drug Administration","the National Key Research and Development Program of China","NIFDC Fund for Key Technology Research","Key Project of NMPA Scientific System Construction for Drug Regulation"],"pagination":["3763"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12472803"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["30(18)"],"pubmed_abstract":["Due to morphological similarities, adulterants are frequently substituted for Qingfengteng (QFT, <i>Sinomenium acutum</i>) in regional markets. This study developed a TaqMan probe-based real-time quantitative polymerase chain reaction (qPCR) assay targeting a 57-base pair (bp) fragment of the internal transcribed spacer 2 (ITS2) region for the specific detection of QFT. The method was validated using a diverse set of samples, including: (1) 19 batches of QFT and 8 batches of Beidougen (BDG, <i>Menispermum dauricum</i>), comprising both medicinal materials and decoction pieces; (2) 5 batches each of decoction pieces from Qingsheteng (QST, <i>Periploca calophylla</i>), Jishiteng (JST, <i>Paederia foetida</i>), Kuanjinteng (KJT, <i>Tinospora sinensis</i>), and Huibeiqingfengteng (HBQFT, <i>Sa"],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Development of TaqMan Real-Time Fluorescent Quantitative PCR Method for Identification and Quantification of &lt;i&gt;Sinomenium acutum-&lt;/i&gt;Originated Herbal Drugs."],"pmcid":["PMC12472803"],"funding_grant_id":["NMPAJGKX-2024","AHYJ-KJ-202402","2023YFC3504104","GJJS-2022-7-2"],"pubmed_authors":["Zhang W","Li X","Tao Y","Zhang Y","Wei F","Duan S","Yu K","Cheng X"],"additional_accession":[]},"is_claimable":false,"name":"Development of TaqMan Real-Time Fluorescent Quantitative PCR Method for Identification and Quantification of &lt;i&gt;Sinomenium acutum-&lt;/i&gt;Originated Herbal Drugs.","description":"Due to morphological similarities, adulterants are frequently substituted for Qingfengteng (QFT, <i>Sinomenium acutum</i>) in regional markets. This study developed a TaqMan probe-based real-time quantitative polymerase chain reaction (qPCR) assay targeting a 57-base pair (bp) fragment of the internal transcribed spacer 2 (ITS2) region for the specific detection of QFT. The method was validated using a diverse set of samples, including: (1) 19 batches of QFT and 8 batches of Beidougen (BDG, <i>Menispermum dauricum</i>), comprising both medicinal materials and decoction pieces; (2) 5 batches each of decoction pieces from Qingsheteng (QST, <i>Periploca calophylla</i>), Jishiteng (JST, <i>Paederia foetida</i>), Kuanjinteng (KJT, <i>Tinospora sinensis</i>), and Huibeiqingfengteng (HBQFT, <i>Sa","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-02T03:12:18.657Z","creation":"2026-05-02T03:08:09.416Z"},"accession":"S-EPMC12472803","cross_references":{"pubmed":["41011655"],"doi":["10.3390/molecules30183763"]}}