biostudies-literatureTao YKey Project of Regulatory Science Research of Anhui Drug Administrationthe National Key Research and Development Program of ChinaNIFDC Fund for Key Technology ResearchKey Project of NMPA Scientific System Construction for Drug Regulation3763https://www.ebi.ac.uk/biostudies/studies/S-EPMC12472803biostudies-literatureUnknown30(18)Due to morphological similarities, adulterants are frequently substituted for Qingfengteng (QFT, <i>Sinomenium acutum</i>) in regional markets. This study developed a TaqMan probe-based real-time quantitative polymerase chain reaction (qPCR) assay targeting a 57-base pair (bp) fragment of the internal transcribed spacer 2 (ITS2) region for the specific detection of QFT. The method was validated using a diverse set of samples, including: (1) 19 batches of QFT and 8 batches of Beidougen (BDG, <i>Menispermum dauricum</i>), comprising both medicinal materials and decoction pieces; (2) 5 batches each of decoction pieces from Qingsheteng (QST, <i>Periploca calophylla</i>), Jishiteng (JST, <i>Paederia foetida</i>), Kuanjinteng (KJT, <i>Tinospora sinensis</i>), and Huibeiqingfengteng (HBQFT, <i>SaMolecules (Basel, Switzerland)Development of TaqMan Real-Time Fluorescent Quantitative PCR Method for Identification and Quantification of &lt;i&gt;Sinomenium acutum-&lt;/i&gt;Originated Herbal Drugs.PMC12472803NMPAJGKX-2024AHYJ-KJ-2024022023YFC3504104GJJS-2022-7-2Zhang WLi XTao YZhang YWei FDuan SYu KCheng XfalseDevelopment of TaqMan Real-Time Fluorescent Quantitative PCR Method for Identification and Quantification of &lt;i&gt;Sinomenium acutum-&lt;/i&gt;Originated Herbal Drugs.Due to morphological similarities, adulterants are frequently substituted for Qingfengteng (QFT, <i>Sinomenium acutum</i>) in regional markets. This study developed a TaqMan probe-based real-time quantitative polymerase chain reaction (qPCR) assay targeting a 57-base pair (bp) fragment of the internal transcribed spacer 2 (ITS2) region for the specific detection of QFT. The method was validated using a diverse set of samples, including: (1) 19 batches of QFT and 8 batches of Beidougen (BDG, <i>Menispermum dauricum</i>), comprising both medicinal materials and decoction pieces; (2) 5 batches each of decoction pieces from Qingsheteng (QST, <i>Periploca calophylla</i>), Jishiteng (JST, <i>Paederia foetida</i>), Kuanjinteng (KJT, <i>Tinospora sinensis</i>), and Huibeiqingfengteng (HBQFT, <i>Sa2025-01-01T00:00:00Z2025 Sep2026-05-02T03:12:18.657Z2026-05-02T03:08:09.416ZS-EPMC124728034101165510.3390/molecules30183763