biostudies-literatureLi RZhongchen MaYong Wang2031https://www.ebi.ac.uk/biostudies/studies/S-EPMC12472996biostudies-literatureUnknown13(9)<i>Mycoplasma bovis</i> (<i>M. bovis</i>) adheres to host cells and persists intracellularly, causing chronic inflammation and significant economic losses in the cattle industry. The role of host cell apoptosis in this host-pathogen interaction remains unclear. This study isolated and identified the <i>M. bovis</i> Xinjiang strain XJ01 from diseased cattle in China. XJ01 exhibited typical "fried egg" colony morphology, distinct biochemical characteristics, and a 1.02 Mb genome (29.33% GC content) encoding 939 genes, including 93 unique genes. Functional analysis under optimal infection conditions (MOI = 1000, 24 h) revealed that XJ01 induced significant apoptosis and reduced viability in bovine macrophages (BoMac). This was accompanied by mitochondrial homeostasis disruption, characterizedMicroorganisms<i>Mycoplasma bovis</i> Infection Induces Apoptosis Through Gadd45/XIAP in Bovine Macrophages.PMC12472996RCZK2024562024AB034Sheng JZhang HMa ZLi RTang TChen CWang YYu Xfalse<i>Mycoplasma bovis</i> Infection Induces Apoptosis Through Gadd45/XIAP in Bovine Macrophages.<i>Mycoplasma bovis</i> (<i>M. bovis</i>) adheres to host cells and persists intracellularly, causing chronic inflammation and significant economic losses in the cattle industry. The role of host cell apoptosis in this host-pathogen interaction remains unclear. This study isolated and identified the <i>M. bovis</i> Xinjiang strain XJ01 from diseased cattle in China. XJ01 exhibited typical "fried egg" colony morphology, distinct biochemical characteristics, and a 1.02 Mb genome (29.33% GC content) encoding 939 genes, including 93 unique genes. Functional analysis under optimal infection conditions (MOI = 1000, 24 h) revealed that XJ01 induced significant apoptosis and reduced viability in bovine macrophages (BoMac). This was accompanied by mitochondrial homeostasis disruption, characterized2025-01-01T00:00:00Z2025 Aug2026-05-02T03:21:33.098Z2026-05-02T03:11:52.01ZS-EPMC124729964101136310.3390/microorganisms13092031