biostudies-literatureUnknown17(9)Alonso-Palomares LAMayaro virus (MAYV) is an alphavirus transmitted by mosquito vectors. Among the three MAYV genotypes (D, L, and N), genotype D has the broadest geographical distribution in Latin America and the Caribbean. The virus can be transmitted by the <i>Aedes</i>, <i>Anopheles</i>, and <i>Haemagogus</i> mosquitoes. To explore the potential expansion of MAYV across the Atlantic Ocean, we compared MAYV (D) infection kinetics in Floridian <i>Aedes</i> aegypti with New World (<i>Anopheles albimanus</i>) and Old World (<i>Anopheles gambiae</i>) anophelines. MAYV infection of both <i>An. albimanus</i> and <i>An. gambiae</i> was rapid, resulting in a higher dissemination rate than <i>Ae. aegypti</i>. We detected MAYV in saliva from <i>An. albimanus</i> (16.6% transmission rate) as early as 2 days post-infection (dpi), increasing to 60% after 7 dpi, a phenomenon (2 dpi) that has not been described to date for mosquitoes. We observed similar increases in MAYV infection of the ovaries and noted marked differences in fecundity for each species tested. Although MAYV infection in <i>An. gambiae</i> was rapid, mosquito lifespan was significantly reduced as compared with both <i>Ae. aegypti</i> and <i>An. albimanus</i>. We discuss the implications of our observations on MAYV transmission risk in Africa by <i>An. gambiae</i> and in the Caribbean and Central America by <i>An. albimanus</i>.Viruses1155https://www.ebi.ac.uk/biostudies/studies/S-EPMC12474012biostudies-literaturePhysiological Impacts on the Mosquito Vector Hosts Refine Vectorial Capacity Estimates of Mayaro Virus Transmission Risk.PMC12474012Burgess ERWilliams JFDinglasan RRAlonso-Palomares LALednicky JAfalsePhysiological Impacts on the Mosquito Vector Hosts Refine Vectorial Capacity Estimates of Mayaro Virus Transmission Risk.Mayaro virus (MAYV) is an alphavirus transmitted by mosquito vectors. Among the three MAYV genotypes (D, L, and N), genotype D has the broadest geographical distribution in Latin America and the Caribbean. The virus can be transmitted by the <i>Aedes</i>, <i>Anopheles</i>, and <i>Haemagogus</i> mosquitoes. To explore the potential expansion of MAYV across the Atlantic Ocean, we compared MAYV (D) infection kinetics in Floridian <i>Aedes</i> aegypti with New World (<i>Anopheles albimanus</i>) and Old World (<i>Anopheles gambiae</i>) anophelines. MAYV infection of both <i>An. albimanus</i> and <i>An. gambiae</i> was rapid, resulting in a higher dissemination rate than <i>Ae. aegypti</i>. We detected MAYV in saliva from <i>An. albimanus</i> (16.6% transmission rate) as early as 2 days post-infection (dpi), increasing to 60% after 7 dpi, a phenomenon (2 dpi) that has not been described to date for mosquitoes. We observed similar increases in MAYV infection of the ovaries and noted marked differences in fecundity for each species tested. Although MAYV infection in <i>An. gambiae</i> was rapid, mosquito lifespan was significantly reduced as compared with both <i>Ae. aegypti</i> and <i>An. albimanus</i>. We discuss the implications of our observations on MAYV transmission risk in Africa by <i>An. gambiae</i> and in the Caribbean and Central America by <i>An. albimanus</i>.2025-01-01T00:00:00Z2025 Aug2026-05-02T03:09:47.83Z2026-05-02T03:07:37.835ZS-EPMC124740124101258310.3390/v17091155