biostudies-literatureSarchese VMinistero della SaluteMinistero dell'università e della ricerca814https://www.ebi.ac.uk/biostudies/studies/S-EPMC12474093biostudies-literatureUnknown12(9)Wild carnivores can harbor pathogens affecting wildlife conservation and domestic animal health. This study surveyed major viral pathogens in free-ranging wolves, red foxes, stone martens, and Eurasian badgers in Northwestern Italy. Duodenal samples from 140 carcasses were screened by consensus PCR for members of the species <i>Protoparvovirus carnivoran1</i> and for canine adenoviruses (CAdV-1/2). PCR-positive samples underwent sequence-independent amplification and Oxford Nanopore sequencing. Canine parvovirus type 2 (CPV-2) and feline panleukopenia virus (FPV) DNAs were identified in three wolves (6.4%) and one badger (4.3%), whereas CAdV-1 was detected in one red fox (1.8%). Nanopore sequencing yielded near-complete genomes of two CPV-new 2a, one CPV-2c, and one FPV strains, along withVeterinary sciencesMolecular Survey for Major Canine Enteric Viral Pathogens in Wild Carnivores, Northwestern Italy.PMC124740932022EPP2TTIZS PLV 08/22 Ricerca CorrenteRobetto SVuillermoz BMarsilio FMartella VDi Profio FOrusa RPellegrini FSarchese VDi Martino BfalseMolecular Survey for Major Canine Enteric Viral Pathogens in Wild Carnivores, Northwestern Italy.Wild carnivores can harbor pathogens affecting wildlife conservation and domestic animal health. This study surveyed major viral pathogens in free-ranging wolves, red foxes, stone martens, and Eurasian badgers in Northwestern Italy. Duodenal samples from 140 carcasses were screened by consensus PCR for members of the species <i>Protoparvovirus carnivoran1</i> and for canine adenoviruses (CAdV-1/2). PCR-positive samples underwent sequence-independent amplification and Oxford Nanopore sequencing. Canine parvovirus type 2 (CPV-2) and feline panleukopenia virus (FPV) DNAs were identified in three wolves (6.4%) and one badger (4.3%), whereas CAdV-1 was detected in one red fox (1.8%). Nanopore sequencing yielded near-complete genomes of two CPV-new 2a, one CPV-2c, and one FPV strains, along with2025-01-01T00:00:00Z2025 Aug2026-05-02T03:20:56.523Z2026-05-02T03:11:48.773ZS-EPMC124740934101274010.3390/vetsci12090814