{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Falk NW"],"funding":["Natural Sciences and Engineering Research Council of Canada"],"pagination":["e70205"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12475508"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(5)"],"pubmed_abstract":["Chronic non-point nitrogen (N) and phosphorus (P) loads reshape sediment microbial biogeochemical cycling in headwater systems, altering ecosystem function. This study integrates DNA and RNA amplicon sequencing with metatranscriptomics to examine microbial taxonomic and functional responses to nutrient inputs in lower Great Lakes watersheds, focusing on N, P, and sulphur (S) metabolism. RNA-based taxa showed a stronger correlation with metabolic functions than DNA-based taxa, highlighting RNA-based approaches as valuable tools for assessing active microbial responses to nutrients. Site-specific analyses revealed distinct microbial metabolic profiles linked to watershed fertiliser sources and seasonal variation. Inorganic fertiliser inputs were associated with tightly coupled N reduction an"],"journal":["Environmental microbiology reports"],"pubmed_title":["Combined DNA/RNA Amplicon Sequencing and Metatranscriptomics Reveals Microbial-Driven Nutrient Transformations and Core Taxa in Agriculturally Impacted Sediments."],"pmcid":["PMC12475508"],"funding_grant_id":["STP 521430‐18","STP 521430-18"],"pubmed_authors":["Drouillard KG","Falk NW","Droppo IG","Weisener CG"],"additional_accession":[]},"is_claimable":false,"name":"Combined DNA/RNA Amplicon Sequencing and Metatranscriptomics Reveals Microbial-Driven Nutrient Transformations and Core Taxa in Agriculturally Impacted Sediments.","description":"Chronic non-point nitrogen (N) and phosphorus (P) loads reshape sediment microbial biogeochemical cycling in headwater systems, altering ecosystem function. This study integrates DNA and RNA amplicon sequencing with metatranscriptomics to examine microbial taxonomic and functional responses to nutrient inputs in lower Great Lakes watersheds, focusing on N, P, and sulphur (S) metabolism. RNA-based taxa showed a stronger correlation with metabolic functions than DNA-based taxa, highlighting RNA-based approaches as valuable tools for assessing active microbial responses to nutrients. Site-specific analyses revealed distinct microbial metabolic profiles linked to watershed fertiliser sources and seasonal variation. Inorganic fertiliser inputs were associated with tightly coupled N reduction an","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-04T01:38:14.591Z","creation":"2026-05-04T03:12:12.227Z"},"accession":"S-EPMC12475508","cross_references":{"pubmed":["41013990"],"doi":["10.1111/1758-2229.70205"]}}