{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["27"],"submitter":["Sicheri E"],"pubmed_abstract":["The projection of conservation onto the surface of a protein's 3D structure is a powerful way of inferring functionally important regions. For this reason, we created ProteoSync, a Python program that semi-automates the process. The program creates an annotated sequence alignment of orthologs from a diverse set of selectable species and enables the fast projection of amino acid conservation onto a predicted or known 3D model in PyMOL <sup>1</sup>. As a test case, we used ProteoSync to analyze a subset of 31 F-box proteins, which function as substrate recognition subunits for a large family of Cul1-based E3 ubiquitin ligases. We correctly identified known substrate interaction surfaces for 11 F-box members with previously solved structures. We also identified likely ligand binding sites for"],"journal":["Computational and structural biotechnology journal"],"pagination":["4026-4039"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12475580"],"repository":["biostudies-literature"],"pubmed_title":["Analysis of Fbox substrate adapter proteins using &lt;i&gt;ProteoSync&lt;/i&gt;, a program for projection of evolutionary conservation onto protein atomic coordinates."],"pmcid":["PMC12475580"],"pubmed_authors":["Tyers M","Mao D","Sicheri E","Sicheri F"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of Fbox substrate adapter proteins using &lt;i&gt;ProteoSync&lt;/i&gt;, a program for projection of evolutionary conservation onto protein atomic coordinates.","description":"The projection of conservation onto the surface of a protein's 3D structure is a powerful way of inferring functionally important regions. For this reason, we created ProteoSync, a Python program that semi-automates the process. The program creates an annotated sequence alignment of orthologs from a diverse set of selectable species and enables the fast projection of amino acid conservation onto a predicted or known 3D model in PyMOL <sup>1</sup>. As a test case, we used ProteoSync to analyze a subset of 31 F-box proteins, which function as substrate recognition subunits for a large family of Cul1-based E3 ubiquitin ligases. We correctly identified known substrate interaction surfaces for 11 F-box members with previously solved structures. We also identified likely ligand binding sites for","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-03T23:51:36.943Z","creation":"2026-05-03T03:11:16.107Z"},"accession":"S-EPMC12475580","cross_references":{"pubmed":["41017827"],"doi":["10.1016/j.csbj.2025.09.012"]}}