biostudies-literatureKosho MXFORANGEForce project within the ORANGEHealth consortium funded by Health-Holland.nl, div. Life Sciences & Health with grant number LSHM21064Philips Oral HealthcareTKI HealthHollandDutch Society of Periodontology (NVvP)Sunstar Suisse S.Amaterial support by Labonovum BV762-774https://www.ebi.ac.uk/biostudies/studies/S-EPMC12476085biostudies-literatureUnknown60(8)<h4>Aims</h4>To explore the potential of metabolomic profiles of oral rinse samples to distinguish between patients with severe periodontitis (stage III/IV) and non-periodontitis controls. This is coupled to an analysis of differences in metabolomic profiles between individuals without periodontitis, patients with localized periodontitis, and patients with generalized periodontitis.<h4>Methods</h4>Periodontitis patients and controls were recruited, all aged ≥ 40 years. Study participants were asked to rinse vigorously for 30 s with 10 mL phosphate buffered saline. Metabolites were identified using a semi-targeted liquid chromatography tandem mass spectrometry (LC-MS/MS) platform.<h4>Results</h4>In total, 38 periodontitis patients (18 localized, 20 generalized stage III/IV periodontitis patJournal of periodontal researchMetabolomic Profiles of Oral Rinse Samples to Distinguish Severe Periodontitis Patients From Non-Periodontitis Controls.PMC12476085LSHM17003Neefjes JGiera MLoos BGCiurli AKosho MXFfalseMetabolomic Profiles of Oral Rinse Samples to Distinguish Severe Periodontitis Patients From Non-Periodontitis Controls.<h4>Aims</h4>To explore the potential of metabolomic profiles of oral rinse samples to distinguish between patients with severe periodontitis (stage III/IV) and non-periodontitis controls. This is coupled to an analysis of differences in metabolomic profiles between individuals without periodontitis, patients with localized periodontitis, and patients with generalized periodontitis.<h4>Methods</h4>Periodontitis patients and controls were recruited, all aged ≥ 40 years. Study participants were asked to rinse vigorously for 30 s with 10 mL phosphate buffered saline. Metabolites were identified using a semi-targeted liquid chromatography tandem mass spectrometry (LC-MS/MS) platform.<h4>Results</h4>In total, 38 periodontitis patients (18 localized, 20 generalized stage III/IV periodontitis pat2025-01-01T00:00:00Z2025 Aug2026-06-04T01:37:44.924Z2026-05-04T03:12:04.266ZS-EPMC124760854008324110.1111/jre.13379