biostudies-literatureUnknown66(12)Pulasani S<h4>Purpose</h4>Pseudoexfoliation (PEX) glaucoma is caused by progressive accumulation of abnormal fibrillar aggregates in aqueous humor outflow tissues. The current lack of effective models for PEX research, replicating the key feature of PEX material production, represents a critical gap in understanding the molecular pathomechanisms and identifying specific therapeutic targets. We have developed an advanced in vitro model using stromal fibroblasts from iridectomy specimens of PEX glaucoma patients with an appropriate genetic background.<h4>Methods</h4>Peripheral iridectomy specimens were obtained from patients with PEX glaucoma (n = 35) during routine trabeculectomy. Stromal fibroblasts were enriched from heterogenous cell cultures. Extracellular matrix formation was stimulated and moduInvestigative ophthalmology & visual science61https://www.ebi.ac.uk/biostudies/studies/S-EPMC12476155biostudies-literatureEstablishment of an Advanced In Vitro Model for Pseudoexfoliation Syndrome and Glaucoma.PMC12476155Lammer RSchlotzer-Schrehardt UPasutto FJunemann AKorn KKruse FEPulasani SZenkel MGießl AfalseEstablishment of an Advanced In Vitro Model for Pseudoexfoliation Syndrome and Glaucoma.<h4>Purpose</h4>Pseudoexfoliation (PEX) glaucoma is caused by progressive accumulation of abnormal fibrillar aggregates in aqueous humor outflow tissues. The current lack of effective models for PEX research, replicating the key feature of PEX material production, represents a critical gap in understanding the molecular pathomechanisms and identifying specific therapeutic targets. We have developed an advanced in vitro model using stromal fibroblasts from iridectomy specimens of PEX glaucoma patients with an appropriate genetic background.<h4>Methods</h4>Peripheral iridectomy specimens were obtained from patients with PEX glaucoma (n = 35) during routine trabeculectomy. Stromal fibroblasts were enriched from heterogenous cell cultures. Extracellular matrix formation was stimulated and modu2025-01-01T00:00:00Z2025 Sep2026-07-15T09:45:37.657Z2026-07-02T03:12:10.919ZS-EPMC124761554099627710.1167/iovs.66.12.61