biostudies-literatureUnknown11Zheng-Qiang LMicroplastics (MPs) are widely distributed and accumulated in the environment, making it nearly impossible for humans to avoid ingestion. Their toxicity can cause serious health damage and pose a threat to human health. In this context, developing strategies to prevent and restore toxic damage from their ingestion is extremely urgent. This study comprehensively employs various techniques, including metagenomics and metabolomics, to explore the pre-protective and restorative effects of <i>Clostridium dalinum</i>, a potential probiotic with excellent antioxidant and anti-inflammatory capabilities, on damage induced by exposure to polystyrene microplastics (PS-MPs) in mice. The results show that exposure to PS-MPs leads to significant intestinal damage in mice; preemptive intake of <i>C. daliCurrent research in food science101200https://www.ebi.ac.uk/biostudies/studies/S-EPMC12477946biostudies-literatureA probiotic for preventing microplastic toxicity: &lt;i&gt;Clostridium dalinum&lt;/i&gt; mitigates microplastic-induced damage via microbiota-metabolism-barrier interactions.PMC12477946Ping MWen XZheng-Qiang LRong SXiao-Yan YJun LWei DRui LRui AXu YfalseA probiotic for preventing microplastic toxicity: &lt;i&gt;Clostridium dalinum&lt;/i&gt; mitigates microplastic-induced damage via microbiota-metabolism-barrier interactions.Microplastics (MPs) are widely distributed and accumulated in the environment, making it nearly impossible for humans to avoid ingestion. Their toxicity can cause serious health damage and pose a threat to human health. In this context, developing strategies to prevent and restore toxic damage from their ingestion is extremely urgent. This study comprehensively employs various techniques, including metagenomics and metabolomics, to explore the pre-protective and restorative effects of <i>Clostridium dalinum</i>, a potential probiotic with excellent antioxidant and anti-inflammatory capabilities, on damage induced by exposure to polystyrene microplastics (PS-MPs) in mice. The results show that exposure to PS-MPs leads to significant intestinal damage in mice; preemptive intake of <i>C. dali2025-01-01T00:00:00Z20252026-06-03T23:26:01.86Z2026-05-03T03:11:04.914ZS-EPMC124779464103038710.1016/j.crfs.2025.101200