{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hussain S"],"funding":["National Institute on Deafness and Other Communication Disorders (NIDCD) Intramural Research Program"],"pagination":["33660"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12480455"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["The hair bundle of auditory and vestibular hair cells converts mechanical stimuli into electrical signals through mechanoelectrical transduction (MET). The MET apparatus is built around a tip link that connects neighboring stereocilia that are aligned in the direction of mechanosensitivity of the hair bundle. Upon stimulation, the MET channel complex responds to changes in tip-link tension and allows a cation influx into the cell. Ca<sup>2+</sup> influx in stereocilia has been used as a signature of MET activity. Using genetically encoded Ca<sup>2+</sup> sensors (GCaMP3, GCaMP6s) and high-performance fluorescence confocal microscopy, we detect spontaneous Ca<sup>2+</sup> transients in individual stereocilia in developing and fully formed hair bundles. We demonstrate that this activity is a"],"journal":["Scientific reports"],"pubmed_title":["Spontaneous calcium transients in hair cell stereocilia."],"pmcid":["PMC12480455"],"funding_grant_id":["Z01-DC000002","1ZIADC000085-01"],"pubmed_authors":["Sedlacek M","Kachar B","Hussain S","Bergles DE","Shin JB","Kindt KS","Zhang-Hooks W","Cui R"],"additional_accession":[]},"is_claimable":false,"name":"Spontaneous calcium transients in hair cell stereocilia.","description":"The hair bundle of auditory and vestibular hair cells converts mechanical stimuli into electrical signals through mechanoelectrical transduction (MET). The MET apparatus is built around a tip link that connects neighboring stereocilia that are aligned in the direction of mechanosensitivity of the hair bundle. Upon stimulation, the MET channel complex responds to changes in tip-link tension and allows a cation influx into the cell. Ca<sup>2+</sup> influx in stereocilia has been used as a signature of MET activity. Using genetically encoded Ca<sup>2+</sup> sensors (GCaMP3, GCaMP6s) and high-performance fluorescence confocal microscopy, we detect spontaneous Ca<sup>2+</sup> transients in individual stereocilia in developing and fully formed hair bundles. We demonstrate that this activity is a","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-04T05:09:27.139Z","creation":"2026-05-05T03:12:46.379Z"},"accession":"S-EPMC12480455","cross_references":{"pubmed":["41022886"],"doi":["10.1038/s41598-025-17976-1"]}}