{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(1)"],"submitter":["Blumenkrantz M"],"pubmed_abstract":["Adult mammalian synovial joints have limited regenerative capacity, where injuries heal with mechanically inferior fibrotic tissues. Here we developed a unilateral whole-joint resection model in adult zebrafish to advance our understanding of how to stimulate regrowth of native synovial joint tissues. Using a combination of microCT, histological, live imaging, and single-cell RNA sequencing (scRNAseq) approaches after complete removal of all joint tissues, we find de novo regeneration of articular cartilage, ligament, and synovium into a functional joint. Clonal lineage tracing and scRNAseq implicate a multipotent, neural crest-derived population in the adult skeleton as a cell source for these regenerating tissues. Together, our findings reveal latent molecular and cellular programs within the adult skeleton that are deployed to regenerate a complex joint with lubricated articular cartilage."],"journal":["Nature communications"],"pagination":["8570"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12480486"],"repository":["biostudies-literature"],"pubmed_title":["Dynamic cell fate plasticity and tissue reintegration drive functional adult synovial joint regeneration after complete resection."],"pmcid":["PMC12480486"],"pubmed_authors":["Gagarin E","Gwin M","Chahine NO","Blumenkrantz M","Kamel MH","Mo J","Sherwood D","Anderson T","Geras A","Dumitrascu B","Smeeton J","Campos L","Woron F","Weinstein E"],"additional_accession":[]},"is_claimable":false,"name":"Dynamic cell fate plasticity and tissue reintegration drive functional adult synovial joint regeneration after complete resection.","description":"Adult mammalian synovial joints have limited regenerative capacity, where injuries heal with mechanically inferior fibrotic tissues. Here we developed a unilateral whole-joint resection model in adult zebrafish to advance our understanding of how to stimulate regrowth of native synovial joint tissues. Using a combination of microCT, histological, live imaging, and single-cell RNA sequencing (scRNAseq) approaches after complete removal of all joint tissues, we find de novo regeneration of articular cartilage, ligament, and synovium into a functional joint. Clonal lineage tracing and scRNAseq implicate a multipotent, neural crest-derived population in the adult skeleton as a cell source for these regenerating tissues. Together, our findings reveal latent molecular and cellular programs within the adult skeleton that are deployed to regenerate a complex joint with lubricated articular cartilage.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-04T05:09:19.112Z","creation":"2026-05-05T03:12:45.549Z"},"accession":"S-EPMC12480486","cross_references":{"pubmed":["41022785"],"doi":["10.1038/s41467-025-63596-8"]}}