{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Subramaniyan I"],"funding":["National Cancer Institute (NCI)","Cancer Prevention Research Institute of Texas Core Facilities Support Award (CPRIT)"],"pagination":["e70274"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12480644"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["48(10)"],"pubmed_abstract":["Structurally similar oxysterols such as 7α-hydroxycholesterol, 7β-hydroxycholesterol, and 7-ketocholesterol; 5,6α- and 5,6β-epoxycholesterol; and 24(R/S)-hydroxy cholesterol, 25-hydroxy cholesterol, and 27-hydroxycholesterol are traditionally difficult to resolve using reversed-phase liquid chromatography (RPLC). We present a simple yet highly optimized method for the simultaneous quantification of eight oxysterols using RPLC coupled with mass spectrometry (MS) without derivatization. Optimal separation of most oxysterols was achieved at a lower column temperature (25°C), with specific combinations of stationary and mobile phases enhancing resolution, particularly for isomeric pairs such as 7α-/7β-OHC, 5,6α-/5,6β-EC, 24 R/S-OHC, and 25-OHC. Although certain analytes (e.g., 24S-OHC and 27-O"],"journal":["Journal of separation science"],"pubmed_title":["Identifying Oxysterols Associated With Age and Diet in Mice Using Optimized Reversed-phase Liquid Chromatography-Mass Spectrometry (RPLC-MS)."],"pmcid":["PMC12480644"],"funding_grant_id":["RP210209","R01CA282339"],"pubmed_authors":["Gollahon L","La-Beck NM","Subramaniyan I","Barr B","Li L","Janesko BG"],"additional_accession":[]},"is_claimable":false,"name":"Identifying Oxysterols Associated With Age and Diet in Mice Using Optimized Reversed-phase Liquid Chromatography-Mass Spectrometry (RPLC-MS).","description":"Structurally similar oxysterols such as 7α-hydroxycholesterol, 7β-hydroxycholesterol, and 7-ketocholesterol; 5,6α- and 5,6β-epoxycholesterol; and 24(R/S)-hydroxy cholesterol, 25-hydroxy cholesterol, and 27-hydroxycholesterol are traditionally difficult to resolve using reversed-phase liquid chromatography (RPLC). We present a simple yet highly optimized method for the simultaneous quantification of eight oxysterols using RPLC coupled with mass spectrometry (MS) without derivatization. Optimal separation of most oxysterols was achieved at a lower column temperature (25°C), with specific combinations of stationary and mobile phases enhancing resolution, particularly for isomeric pairs such as 7α-/7β-OHC, 5,6α-/5,6β-EC, 24 R/S-OHC, and 25-OHC. Although certain analytes (e.g., 24S-OHC and 27-O","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-04T00:00:04.941Z","creation":"2026-05-03T03:11:56.802Z"},"accession":"S-EPMC12480644","cross_references":{"pubmed":["41024374"],"doi":["10.1002/jssc.70274"]}}