{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xie J"],"funding":["Japan Agency for Medical Research and Development","Takeda Science Foundation; and performed as research programmes of the Grant-in-Aid for Scientific Research, the Japan Society for the Promotion of Science"],"pagination":["251-265"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12480733"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["178(4)"],"pubmed_abstract":["Long non-coding RNAs (lncRNAs) regulate a wide array of cellular processes through interactions with RNA-binding proteins (RBPs). Taurine Upregulated Gene 1 (TUG1) is an lncRNA that is overexpressed in many types of cancer and has been implicated in resolving R-loops, thereby maintaining genomic integrity. However, the full spectrum of its protein interactions and stress-responsive dynamics remains unclear. Here, we employed CRISPR-assisted RNA-protein interaction detection (CARPID) combined with mass spectrometry to comprehensively identify the interacting proteins of TUG1 in HEK293T cells. Using three distinct single-guide RNAs (sgRNAs) targeting different regions of TUG1, we consistently identified 17 TUG1-interacting proteins under basal conditions. Upon camptothecin (CPT) treatment, w"],"journal":["Journal of biochemistry"],"pubmed_title":["Comprehensive identification of proteins interacting with long non-coding RNA TUG1 in R-loop regulation."],"pmcid":["PMC12480733"],"funding_grant_id":["23H02747","23ck0106816h0001 and 23ama221204h0002","23K06634"],"pubmed_authors":["Watanabe S","Ito T","Kondo Y","Nakagawa R","Xie J","Suzuki MM","Shinjo K","Iijima K","Nishimura T"],"additional_accession":[]},"is_claimable":false,"name":"Comprehensive identification of proteins interacting with long non-coding RNA TUG1 in R-loop regulation.","description":"Long non-coding RNAs (lncRNAs) regulate a wide array of cellular processes through interactions with RNA-binding proteins (RBPs). Taurine Upregulated Gene 1 (TUG1) is an lncRNA that is overexpressed in many types of cancer and has been implicated in resolving R-loops, thereby maintaining genomic integrity. However, the full spectrum of its protein interactions and stress-responsive dynamics remains unclear. Here, we employed CRISPR-assisted RNA-protein interaction detection (CARPID) combined with mass spectrometry to comprehensively identify the interacting proteins of TUG1 in HEK293T cells. Using three distinct single-guide RNAs (sgRNAs) targeting different regions of TUG1, we consistently identified 17 TUG1-interacting proteins under basal conditions. Upon camptothecin (CPT) treatment, w","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-31T03:12:55.091Z","creation":"2026-05-31T03:09:23.374Z"},"accession":"S-EPMC12480733","cross_references":{"pubmed":["40654306"],"doi":["10.1093/jb/mvaf042"]}}