biostudies-literatureUnknown15Zhu S<h4>Background</h4><i>Salmonella</i> Enteritidis (<i>S</i>. Enteritidis) is an important zoonotic pathogen that poses a major threat to animals and human health. TrxB, as a key component of the thioredoxin system, is a thioredoxin reductase ubiquitously present in organisms. It is mainly involved in maintaining cellular redox balance, but its role in the pathogenicity of <i>S</i>. Enteritidis remains unclear.<h4>Methods</h4>In this study, we generated a <i>trxB</i>-deficient strain from <i>S</i>. Enteritidis C50336 strain to investigate how TrxB affects the biological characteristics and pathogenesis of the bacterium. The virulence of Δ<i>trxB</i> was assessed by measuring Δ<i>trxB</i> resistance to environmental stress, biofilm formation ability, motility, adhesion, invasion ability, intrFrontiers in cellular and infection microbiology1659729https://www.ebi.ac.uk/biostudies/studies/S-EPMC12484166biostudies-literatureCharacterization and immunoprotection of thioredoxin reductase TrxB knockout mutant of &lt;i&gt;Salmonella&lt;/i&gt; Enteritidis.PMC12484166Liu XLi HLi CDong YZhang ZShi QZhu XRen CZhu SWang LfalseCharacterization and immunoprotection of thioredoxin reductase TrxB knockout mutant of &lt;i&gt;Salmonella&lt;/i&gt; Enteritidis.<h4>Background</h4><i>Salmonella</i> Enteritidis (<i>S</i>. Enteritidis) is an important zoonotic pathogen that poses a major threat to animals and human health. TrxB, as a key component of the thioredoxin system, is a thioredoxin reductase ubiquitously present in organisms. It is mainly involved in maintaining cellular redox balance, but its role in the pathogenicity of <i>S</i>. Enteritidis remains unclear.<h4>Methods</h4>In this study, we generated a <i>trxB</i>-deficient strain from <i>S</i>. Enteritidis C50336 strain to investigate how TrxB affects the biological characteristics and pathogenesis of the bacterium. The virulence of Δ<i>trxB</i> was assessed by measuring Δ<i>trxB</i> resistance to environmental stress, biofilm formation ability, motility, adhesion, invasion ability, intr2025-01-01T00:00:00Z20252026-06-04T02:30:03.431Z2026-05-04T03:13:43.972ZS-EPMC124841664104098610.3389/fcimb.2025.1659729