biostudies-literatureZhang WSevero Ochoa Program for Centres of Excellence in R&D'ERDF A way of making Europe'CERCA Program of the Catalan governmentSevero Ochoa Program for Centres of Excellence in R&DMinisterio de Ciencia e Innovacion‘ERDF A way of making Europe’4340-4358https://www.ebi.ac.uk/biostudies/studies/S-EPMC12485366biostudies-literatureUnknown76(15)Bacterial wilt caused by Ralstonia solanacearum is one of the most destructive bacterial diseases for which no effective treatment exists. There is an urgent need to understand the basis of resistance against this pathogen in order to engineer efficient strategies in the field. We previously demonstrated that resistant tomato plants limit bacterial movement in the apoplast and the xylem. As a first step to dissect the underlying mechanisms, we analysed the apoplast proteome upon challenge with R. solanacearum in the susceptible tomato cultivar Marmande and the resistant cultivar Hawaii 7996. Here, we described the xylem proteome in these same cultivars and compared it with the apoplastic proteome, revealing variety-dependent and infection-dependent changes. This proteomic analysis led to tJournal of experimental botanyThe CAPE1 peptide confers resistance against bacterial wilt in tomato.PMC12485366CEX2019-000902-SMCIN/AEI/10.13039/501100011033CSC202408390094CSC202306990037CSC201906990041TED2021-131457B-I00Zhang WTakken FLWKaschani FColl NSValls MPlanas-Marques MVermeulen ALiang MZhang QKaiser MfalseThe CAPE1 peptide confers resistance against bacterial wilt in tomato.Bacterial wilt caused by Ralstonia solanacearum is one of the most destructive bacterial diseases for which no effective treatment exists. There is an urgent need to understand the basis of resistance against this pathogen in order to engineer efficient strategies in the field. We previously demonstrated that resistant tomato plants limit bacterial movement in the apoplast and the xylem. As a first step to dissect the underlying mechanisms, we analysed the apoplast proteome upon challenge with R. solanacearum in the susceptible tomato cultivar Marmande and the resistant cultivar Hawaii 7996. Here, we described the xylem proteome in these same cultivars and compared it with the apoplastic proteome, revealing variety-dependent and infection-dependent changes. This proteomic analysis led to t2025-01-01T00:00:00Z2025 Oct2026-06-04T00:17:41.894Z2026-05-03T03:12:13.375ZS-EPMC124853664017629310.1093/jxb/eraf145