{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13(10)"],"submitter":["Autio M"],"funding":["Finnish Cancer Organizations","Finska Läkaresällskapet (FLS)","University of Helsinki","Biomedicum Helsinki-säätiö (Biomedicum Helsinki Foundation)","Sigrid Juséliuksen Säätiö","Biomedicum Helsinki-säätiö","Suomen Onkologiayhdistys (Finnish Society of Oncology)","Research Council of Finland","Research Council of Finland (AKA)","Suomen Onkologiayhdistys","Helsinki and Uusimaa Hospital District","Sigrid Juséliuksen Säätiö (Sigrid Jusélius Stiftelse)","Finska Läkaresällskapet"],"pubmed_abstract":["Recent studies have explored the composition of the tumor microenvironment (TME) in diffuse large B-cell lymphoma (DLBCL) However, cell-to-cell interactions, along with the spatial organization of DLBCL TME and their impact on patient outcomes, have remained poorly characterized. We applied multiplex immunofluorescence, cell phenotyping, and neighborhood analysis to investigate 1,218,756 single cells in 99 samples from patients with primary DLBCL. We identified 17 cell phenotypes and 10 recurrent cellular neighborhoods (RCN) across samples, subdividing DLBCLs into immune-poor areas and areas with diverse immune cell infiltrates. Avoidance of B cells and PD-1+ T cells was associated with less aggressive clinical characteristics and favorable survival. Likewise, the proximity of CD8+ T cell-"],"journal":["Cancer immunology research"],"pagination":["1674-1686"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12485370"],"repository":["biostudies-literature"],"pubmed_title":["Spatial Analysis of the Tumor Microenvironment in Diffuse Large B-cell Lymphoma Reveals Clinically Relevant Cell Interactions and Recurrent Cellular Neighborhoods."],"pmcid":["PMC12485370"],"pubmed_authors":["Meriranta L","Autio M","Karjalainen-Lindsberg ML","Pellinen T","Leppa S","Leivonen SK"],"additional_accession":[]},"is_claimable":false,"name":"Spatial Analysis of the Tumor Microenvironment in Diffuse Large B-cell Lymphoma Reveals Clinically Relevant Cell Interactions and Recurrent Cellular Neighborhoods.","description":"Recent studies have explored the composition of the tumor microenvironment (TME) in diffuse large B-cell lymphoma (DLBCL) However, cell-to-cell interactions, along with the spatial organization of DLBCL TME and their impact on patient outcomes, have remained poorly characterized. We applied multiplex immunofluorescence, cell phenotyping, and neighborhood analysis to investigate 1,218,756 single cells in 99 samples from patients with primary DLBCL. We identified 17 cell phenotypes and 10 recurrent cellular neighborhoods (RCN) across samples, subdividing DLBCLs into immune-poor areas and areas with diverse immune cell infiltrates. Avoidance of B cells and PD-1+ T cells was associated with less aggressive clinical characteristics and favorable survival. Likewise, the proximity of CD8+ T cell-","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-04T00:30:07.111Z","creation":"2026-05-03T03:12:19.779Z"},"accession":"S-EPMC12485370","cross_references":{"pubmed":["40772779"],"doi":["10.1158/2326-6066.CIR-24-1163"]}}