biostudies-literatureIshimatsu MLotte Research Promotion GrantUehara Memorial FoundationSupport for Pioneering Research Initiated by the Next GenerationJapan Society for the Promotion of Science1643-1657https://www.ebi.ac.uk/biostudies/studies/S-EPMC12485865biostudies-literatureUnknown15(10)Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is a master transcriptional cofactor for mitochondrial biogenesis. Mitochondrial intermediate peptidase (MIPEP), a mitochondrial signal peptidase, plays an important role in the maturation and activation of mitochondrial proteins. Caloric restriction has lifespan-extending effects that are reportedly exerted through induced expression of PGC-1α and MIPEP in white adipose tissue. To evaluate how upregulation of PGC-1α and MIPEP contributes to changes in the cellular characteristics of adipocytes, this study examined the mitochondrial function and differentiation of 3T3-L1 preadipocytes with single overexpression (OE) or double OE of Pgc-1α and Mipep. Compared with single-OE cells, double-OE cells exhibited no signFEBS open bioCo-overexpression of the caloric restriction-induced mitochondrial factors PGC-1α and MIPEP upregulates Phospho1 expression in adipocytes.PMC1248586520K1JPMJSP21517K1323Higami YKato KMizunoe YNarita TTaki KIshimatsu MNozaki YHayami AKobayashi MMori RfalseCo-overexpression of the caloric restriction-induced mitochondrial factors PGC-1α and MIPEP upregulates Phospho1 expression in adipocytes.Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) is a master transcriptional cofactor for mitochondrial biogenesis. Mitochondrial intermediate peptidase (MIPEP), a mitochondrial signal peptidase, plays an important role in the maturation and activation of mitochondrial proteins. Caloric restriction has lifespan-extending effects that are reportedly exerted through induced expression of PGC-1α and MIPEP in white adipose tissue. To evaluate how upregulation of PGC-1α and MIPEP contributes to changes in the cellular characteristics of adipocytes, this study examined the mitochondrial function and differentiation of 3T3-L1 preadipocytes with single overexpression (OE) or double OE of Pgc-1α and Mipep. Compared with single-OE cells, double-OE cells exhibited no sign2025-01-01T00:00:00Z2025 Oct2026-06-04T00:33:35.265Z2026-05-03T03:11:51.872ZS-EPMC124858654057434610.1002/2211-5463.70077