biostudies-literatureHarada NTakeda Science FoundationJapan Agency for Medical Research and DevelopmentHoansha FoundationJapan Science and Technology AgencyJapan Society for the Promotion of Sciencee71286https://www.ebi.ac.uk/biostudies/studies/S-EPMC12486328biostudies-literatureUnknown14(19)<h4>Background</h4>We have previously developed a gold nanoparticle (GNP)-based anti-cancer immunotherapy, termed integrated glyco-nanovaccine (iGN). The iGN is composed of GNPs conjugated to a synthetic toll-like receptor (TLR) 7 ligand, an antigen peptide, and a mannose sugar chain. However, the effect of the combination of different sugar chains and antigen peptides on iGN-mediated anticancer immunotherapy remains to be elucidated.<h4>Objective</h4>We compared the anti-tumor effects of two different sugar chains: α-mannose and sialic acid.<h4>Results</h4>We showed that not only the sugar chain but also the antigen peptide plays a pivotal role in iGN uptake by immune cells. In contrast to α-mannose, which promoted GNP internalization by bone marrow-derived dendritic cells (BMDC), sialic Cancer medicinePeptide Modulation Overrides Glycan Synergy in Gold Nanoparticle-Based Vaccines for Cancer Immunotherapy.PMC12486328JPMJFR226JJP22cm010638224H0086424H00865JPMJPR17HAJP22gm6210030Sakamoto YWada SMoroishi TShimoda MShinchi HMurata KWakao MNiimura MHarada NKamba TNita AfalsePeptide Modulation Overrides Glycan Synergy in Gold Nanoparticle-Based Vaccines for Cancer Immunotherapy.<h4>Background</h4>We have previously developed a gold nanoparticle (GNP)-based anti-cancer immunotherapy, termed integrated glyco-nanovaccine (iGN). The iGN is composed of GNPs conjugated to a synthetic toll-like receptor (TLR) 7 ligand, an antigen peptide, and a mannose sugar chain. However, the effect of the combination of different sugar chains and antigen peptides on iGN-mediated anticancer immunotherapy remains to be elucidated.<h4>Objective</h4>We compared the anti-tumor effects of two different sugar chains: α-mannose and sialic acid.<h4>Results</h4>We showed that not only the sugar chain but also the antigen peptide plays a pivotal role in iGN uptake by immune cells. In contrast to α-mannose, which promoted GNP internalization by bone marrow-derived dendritic cells (BMDC), sialic 2025-01-01T00:00:00Z2025 Oct2026-06-04T01:26:51.527Z2026-05-03T03:14:11.285ZS-EPMC124863284103172510.1002/cam4.71286