{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["32(1)"],"submitter":["Lapmanee S"],"pubmed_abstract":["This study presents the development and evaluation of <i>Centella Asiatica</i> (CA)-loaded transfersomes (CANP) as a novel nanocarrier for transdermal delivery. CANP were prepared using an oil-in-water emulsion method, producing nanoparticles with a size of 135.22 ± 4.80 nm, a polydispersity index of 0.22 ± 0.01, and a zeta potential of -26.13 ± 0.58 mV. Stability tests confirmed consistent physicochemical properties under various storage conditions, with encapsulation efficiencies above 68% for madecassoside and 89% for asiaticoside. <i>Ex vivo</i> permeation studies using porcine skin showed significantly improved skin penetration compared to liposomes and niosomes, attributed to the high deformability index (1.31 ± 0.21 mg/cm<sup>2</sup>). <i>In vitro</i> cytotoxicity assays indicated cell viability above 80% across concentrations. Functionally, CANP reduced nitric oxide production in LPS-stimulated RAW 264.7 cells, demonstrating superior anti-inflammatory effects over native CA. CANP also promoted fibroblast proliferation and collagen production by 91.9% and 213.3% at days 7 and 14, respectively, exceeding vitamin C. Wound healing assays confirmed enhanced fibroblast migration and closure rates similar to fibroblast growth factor. <i>In vivo</i>, CANP hydrogels accelerated healing, with early fibroblast activity and collagen deposition between days 7-14, supporting epithelial regeneration over 21 days. Compared to controls, they more effectively reduced inflammation and increased dermal growth factor expression. These findings support CANP as a promising transdermal nanocarrier with enhanced skin penetration, anti-inflammatory activity, and regenerative potential. Encapsulating CA into transfersomes boosts its therapeutic efficacy, making it a strong candidate for advanced dermal applications."],"journal":["Drug delivery"],"pagination":["2563649"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12486451"],"repository":["biostudies-literature"],"pubmed_title":["Transfersomal delivery of &lt;i&gt;Centella asiatica&lt;/i&gt; promotes efficient excision wound healing in rats."],"pmcid":["PMC12486451"],"pubmed_authors":["Wongchitrat P","Suttisintong K","Phongsupa W","Lapmanee S","Bunwatcharaphansakun P","Khongkow M","Ruktanonchai U","Bhubhanil S","Namdee K","Asawapirom U","Maitarad P"],"additional_accession":[]},"is_claimable":false,"name":"Transfersomal delivery of &lt;i&gt;Centella asiatica&lt;/i&gt; promotes efficient excision wound healing in rats.","description":"This study presents the development and evaluation of <i>Centella Asiatica</i> (CA)-loaded transfersomes (CANP) as a novel nanocarrier for transdermal delivery. CANP were prepared using an oil-in-water emulsion method, producing nanoparticles with a size of 135.22 ± 4.80 nm, a polydispersity index of 0.22 ± 0.01, and a zeta potential of -26.13 ± 0.58 mV. Stability tests confirmed consistent physicochemical properties under various storage conditions, with encapsulation efficiencies above 68% for madecassoside and 89% for asiaticoside. <i>Ex vivo</i> permeation studies using porcine skin showed significantly improved skin penetration compared to liposomes and niosomes, attributed to the high deformability index (1.31 ± 0.21 mg/cm<sup>2</sup>). <i>In vitro</i> cytotoxicity assays indicated cell viability above 80% across concentrations. Functionally, CANP reduced nitric oxide production in LPS-stimulated RAW 264.7 cells, demonstrating superior anti-inflammatory effects over native CA. CANP also promoted fibroblast proliferation and collagen production by 91.9% and 213.3% at days 7 and 14, respectively, exceeding vitamin C. Wound healing assays confirmed enhanced fibroblast migration and closure rates similar to fibroblast growth factor. <i>In vivo</i>, CANP hydrogels accelerated healing, with early fibroblast activity and collagen deposition between days 7-14, supporting epithelial regeneration over 21 days. Compared to controls, they more effectively reduced inflammation and increased dermal growth factor expression. These findings support CANP as a promising transdermal nanocarrier with enhanced skin penetration, anti-inflammatory activity, and regenerative potential. Encapsulating CA into transfersomes boosts its therapeutic efficacy, making it a strong candidate for advanced dermal applications.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-04T00:37:06.602Z","creation":"2026-05-03T03:13:00.303Z"},"accession":"S-EPMC12486451","cross_references":{"pubmed":["41025315"],"doi":["10.1080/10717544.2025.2563649"]}}