biostudies-literatureUnknown25(1)Wang L<h4>Objective</h4>The etiology of obsessive-compulsive disorder (OCD) is multifactorial and remains incompletely understood. While both the Tryptophan hydroxylase 2 (TPH2) gene and the family environment have been implicated in OCD, few studies have examined their combined effects. This study aimed to investigate the association between TPH2 DNA methylation and family functioning and parenting styles in patients with OCD.<h4>Method</h4>A total of 58 individuals with OCD and 89 age- and gender- matched healthy controls were recruited. DNA methylation levels at 12 CpG sites in the promoter region of TPH2 were quantified using the MassARRAY system. OCD symptom severity was assessed using the Yale-Brown Obsessive Compulsive Scale (Y-BOCS). Parenting styles and family functioning were evaluatedBMC psychiatry882https://www.ebi.ac.uk/biostudies/studies/S-EPMC12486529biostudies-literatureInfluence of TPH2 DNA methylation and family functioning, parenting styles on OCD severity.PMC12486529Hu SLiu ZQiao DChen YYu TWang LfalseInfluence of TPH2 DNA methylation and family functioning, parenting styles on OCD severity.<h4>Objective</h4>The etiology of obsessive-compulsive disorder (OCD) is multifactorial and remains incompletely understood. While both the Tryptophan hydroxylase 2 (TPH2) gene and the family environment have been implicated in OCD, few studies have examined their combined effects. This study aimed to investigate the association between TPH2 DNA methylation and family functioning and parenting styles in patients with OCD.<h4>Method</h4>A total of 58 individuals with OCD and 89 age- and gender- matched healthy controls were recruited. DNA methylation levels at 12 CpG sites in the promoter region of TPH2 were quantified using the MassARRAY system. OCD symptom severity was assessed using the Yale-Brown Obsessive Compulsive Scale (Y-BOCS). Parenting styles and family functioning were evaluated2025-01-01T00:00:00Z2025 Sep2026-07-15T06:49:59.331Z2026-06-30T03:22:54.831ZS-EPMC124865294102956010.1186/s12888-025-07217-0