biostudies-literatureUnknown7(1)Ruampatana JThe Second Century Fund (C2F) at Chulalongkorn University, Thailand<h4>Background</h4>Gut microbiota plays a crucial role in swine health and performance, with diet as a key modulator. Euglena gracilis supplementation has shown immunomodulatory effects that benefit sows and piglets. However, its prebiotic effect on gut microbiota remains unclear. This study investigated the impact of maternal E. gracilis supplementation on gut microbiota, piglet body weight, and the incidence of diarrhea.<h4>Methods</h4>Sixty-one crossbred sows (Landrace × Yorkshire) were assigned to either a standard diet (Control; n = 30) or a standard diet supplemented with 1 g/sow/day of E. gracilis (E. gracilis; n = 31) from day 85 of gestation until day 21 of lactation. On day 109 of gestation, 30 sows (15/group) were randomly selected for performance assessment and fecal sample colAnimal microbiome99https://www.ebi.ac.uk/biostudies/studies/S-EPMC12486872biostudies-literatureMaternal Euglena gracilis supplementation alters sow and piglet gut microbiota, reduces diarrhea incidence, and enhances piglet growth.PMC12486872Kamolklang PFeyera TNuntapaitoon MRuampatana JTunsakul NYamsrikaew USomboonna NfalseMaternal Euglena gracilis supplementation alters sow and piglet gut microbiota, reduces diarrhea incidence, and enhances piglet growth.<h4>Background</h4>Gut microbiota plays a crucial role in swine health and performance, with diet as a key modulator. Euglena gracilis supplementation has shown immunomodulatory effects that benefit sows and piglets. However, its prebiotic effect on gut microbiota remains unclear. This study investigated the impact of maternal E. gracilis supplementation on gut microbiota, piglet body weight, and the incidence of diarrhea.<h4>Methods</h4>Sixty-one crossbred sows (Landrace × Yorkshire) were assigned to either a standard diet (Control; n = 30) or a standard diet supplemented with 1 g/sow/day of E. gracilis (E. gracilis; n = 31) from day 85 of gestation until day 21 of lactation. On day 109 of gestation, 30 sows (15/group) were randomly selected for performance assessment and fecal sample col2025-01-01T00:00:00Z2025 Oct2026-06-04T01:41:52.206Z2026-05-04T03:13:09.685ZS-EPMC124868724103500210.1186/s42523-025-00467-z