{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sakhawala RM"],"funding":["Intramural NIH HHS","NIH HHS"],"pagination":["1198-1218"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12487709"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["39(19-20)"],"pubmed_abstract":["MicroRNAs (miRNAs) are short RNAs that post-transcriptionally regulate gene expression. In canonical miRNA biogenesis, primary miRNAs are transcribed from intergenic loci or intronic regions by RNA polymerase II and sequentially cleaved by the Microprocessor complex and Dicer, and the resulting mature miRNAs are loaded into Argonaute to repress target mRNAs. A minority of miRNAs are generated via noncanonical biogenesis pathways that bypass the Microprocessor complex and/or Dicer. Here, we describe a new Pol III-dependent, Microprocessor-independent, and Dicer-dependent biogenesis pathway exemplified by the <i>mir-1829</i> family in <i>Caenorhabditis elegans</i> Although the <i>mir-1829</i> family loci reside in intronic regions of protein-coding genes, we show that the miRNAs are derived "],"journal":["Genes & development"],"pubmed_title":["A noncanonical Pol III-dependent, Microprocessor-independent biogenesis pathway generates a germline-enriched miRNA family."],"pmcid":["PMC12487709"],"funding_grant_id":["ZIA DK075147","P40 OD010440"],"pubmed_authors":["Vieux KF","McJunkin K","Yu G","Sakhawala RM","Haskell D","Zinovyeva A","Tirgar R"],"additional_accession":[]},"is_claimable":false,"name":"A noncanonical Pol III-dependent, Microprocessor-independent biogenesis pathway generates a germline-enriched miRNA family.","description":"MicroRNAs (miRNAs) are short RNAs that post-transcriptionally regulate gene expression. In canonical miRNA biogenesis, primary miRNAs are transcribed from intergenic loci or intronic regions by RNA polymerase II and sequentially cleaved by the Microprocessor complex and Dicer, and the resulting mature miRNAs are loaded into Argonaute to repress target mRNAs. A minority of miRNAs are generated via noncanonical biogenesis pathways that bypass the Microprocessor complex and/or Dicer. Here, we describe a new Pol III-dependent, Microprocessor-independent, and Dicer-dependent biogenesis pathway exemplified by the <i>mir-1829</i> family in <i>Caenorhabditis elegans</i> Although the <i>mir-1829</i> family loci reside in intronic regions of protein-coding genes, we show that the miRNAs are derived ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-07-15T09:12:30.42Z","creation":"2026-07-02T03:08:52.746Z"},"accession":"S-EPMC12487709","cross_references":{"pubmed":["40659526"],"doi":["10.1101/gad.352481.124"]}}