{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gordon MT"],"funding":["National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["2095-2104"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12487726"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(9)"],"pubmed_abstract":["PKAL-1 is a fatty acyl-AMP ligase that plays an essential role in the biosynthesis of the nemamides, hybrid polyketide-nonribosomal peptides from nematodes that promote recovery from starvation-induced larval arrest. This enzyme traffics biosynthetic intermediates between two enzymatic assembly lines: the hybrid polyketide synthase-nonribosomal peptide synthetase PKS-1 and the nonribosomal peptide synthetase NRPS-1. Specifically, a <i>pkal-1</i> mutant worm strain does not produce the nemamides but accumulates a β-amino-containing polyketide, named nematide A, which is made by PKS-1 and is potentially loaded by PKAL-1 onto NRPS-1. Here, however, we show that PKAL-1 does not accept β-amino-containing substrates. Furthermore, phylogenetic and structural analyses of PKAL-1 indicate that it is"],"journal":["ACS chemical biology"],"pubmed_title":["Characterizing the Biochemical Role of an Unusual Fatty Acyl-AMP Ligase from a Hybrid PKS-NRPS Pathway in Nematodes."],"pmcid":["PMC12487726"],"funding_grant_id":["R35 GM114076","R35 GM144076"],"pubmed_authors":["Li X","Priya","Yu R","Gordon MT","Gonzalez MS","Abraham E","Butcher RA","Prajapati DV"],"additional_accession":[]},"is_claimable":false,"name":"Characterizing the Biochemical Role of an Unusual Fatty Acyl-AMP Ligase from a Hybrid PKS-NRPS Pathway in Nematodes.","description":"PKAL-1 is a fatty acyl-AMP ligase that plays an essential role in the biosynthesis of the nemamides, hybrid polyketide-nonribosomal peptides from nematodes that promote recovery from starvation-induced larval arrest. This enzyme traffics biosynthetic intermediates between two enzymatic assembly lines: the hybrid polyketide synthase-nonribosomal peptide synthetase PKS-1 and the nonribosomal peptide synthetase NRPS-1. Specifically, a <i>pkal-1</i> mutant worm strain does not produce the nemamides but accumulates a β-amino-containing polyketide, named nematide A, which is made by PKS-1 and is potentially loaded by PKAL-1 onto NRPS-1. Here, however, we show that PKAL-1 does not accept β-amino-containing substrates. Furthermore, phylogenetic and structural analyses of PKAL-1 indicate that it is","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-04T01:55:09.879Z","creation":"2026-05-04T03:14:10.981Z"},"accession":"S-EPMC12487726","cross_references":{"pubmed":["40814983"],"doi":["10.1021/acschembio.5c00237"]}}