biostudies-literaturePoetzsch SNFoundation for Research in Science and the Humanities2086-2095https://www.ebi.ac.uk/biostudies/studies/S-EPMC12489288biostudies-literatureUnknown17(10)Cannabis sativa L. constituents, such as cannabinoids, terpenes, flavonoids, and other secondary metabolites, determine the plant's (medicinal) effects and properties in a complex interplay, a phenomenon known as the entourage effect. However, environmental influences like cultivation method, soil, light, and climate might also influence the plant's chemical composition-and thus its therapeutic profile. Much like in viticulture, the concept of a "cannabis terroir" might play an important role in determining the plant's chemical phenotype. The aim of this study was therefore to make these complex properties analytically accessible and develop a comprehensive metabolomics workflow using gas chromatography-high-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution tDrug testing and analysisToward a Cannabis Terroir: Untargeted Metabolomic Profiling of Authentic Samples Using Gas Chromatography-High-Resolution Mass Spectrometry (GC-HRMS) and Liquid Chromatography-High-Resolution Tandem Mass Spectrometry (LC-HRMS/MS).PMC12489288STWF-22-012STWF‐22‐012Poetzsch MKraemer TSteuer AEPoetzsch SNfalseToward a Cannabis Terroir: Untargeted Metabolomic Profiling of Authentic Samples Using Gas Chromatography-High-Resolution Mass Spectrometry (GC-HRMS) and Liquid Chromatography-High-Resolution Tandem Mass Spectrometry (LC-HRMS/MS).Cannabis sativa L. constituents, such as cannabinoids, terpenes, flavonoids, and other secondary metabolites, determine the plant's (medicinal) effects and properties in a complex interplay, a phenomenon known as the entourage effect. However, environmental influences like cultivation method, soil, light, and climate might also influence the plant's chemical composition-and thus its therapeutic profile. Much like in viticulture, the concept of a "cannabis terroir" might play an important role in determining the plant's chemical phenotype. The aim of this study was therefore to make these complex properties analytically accessible and develop a comprehensive metabolomics workflow using gas chromatography-high-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution t2025-01-01T00:00:00Z2025 Oct2026-06-04T00:38:20.625Z2026-05-03T03:13:10.943ZS-EPMC124892884058919110.1002/dta.3922