{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(38)"],"submitter":["Thanapaul NB"],"pubmed_abstract":["Rhizobium bacteria convert atmospheric nitrogen into ammonia that leguminous plants can use as a nitrogen source, reducing the need for synthetic nitrogen fertilizers. <i>Bradyrhizobium</i> sp. SUTN9-2 is an effective biofertilizer suitable for legume-rice rotational cropping systems that promotes growth and stress resistance. Deploying this biofertilizer for sustainable agriculture requires a rapid and cost-effective method for monitoring bacterial concentrations in the soil. In this work, we developed and optimized an antibody-based biosensor for the detection of <i>Bradyrhizobium</i> sp. SUTN9-2 via electrochemical impedance spectroscopy (EIS). The immunosensor was fabricated by covalently immobilizing a recombinant antibody specific to SUTN9-2 onto a dithiobis-succinimidyl propionate (DSP) self-assembled monolayer on a gold electrode. The resulting immunosensor effectively captures <i>Bradyrhizobium</i> sp. SUTN9-2 cells are present in samples, leading to changes in electrode impedance. The immunosensor demonstrated excellent specificity, a wide linear detection range of 1 × 10<sup>3</sup> to 1 × 10<sup>7</sup> CFU/mL (<i>R</i> <sup>2</sup> = 0.992), and a limit of detection approaching the single-cell detection limit. The compatibility of the sensor with a soil sample was also demonstrated. The platform holds great promise for the portable monitoring of soil biofertilizer levels and could also be adapted to other strains of interest."],"journal":["ACS omega"],"pagination":["44489-44498"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12489705"],"repository":["biostudies-literature"],"pubmed_title":["Sensitive Detection of Nitrogen-Fixing Soil Bacteria by Impedimetric Immunosensing."],"pmcid":["PMC12489705"],"pubmed_authors":["Yamabhai M","Pinyou P","Blay V","Thanapaul NB","Bista S","Tittabutr P"],"additional_accession":[]},"is_claimable":false,"name":"Sensitive Detection of Nitrogen-Fixing Soil Bacteria by Impedimetric Immunosensing.","description":"Rhizobium bacteria convert atmospheric nitrogen into ammonia that leguminous plants can use as a nitrogen source, reducing the need for synthetic nitrogen fertilizers. <i>Bradyrhizobium</i> sp. SUTN9-2 is an effective biofertilizer suitable for legume-rice rotational cropping systems that promotes growth and stress resistance. Deploying this biofertilizer for sustainable agriculture requires a rapid and cost-effective method for monitoring bacterial concentrations in the soil. In this work, we developed and optimized an antibody-based biosensor for the detection of <i>Bradyrhizobium</i> sp. SUTN9-2 via electrochemical impedance spectroscopy (EIS). The immunosensor was fabricated by covalently immobilizing a recombinant antibody specific to SUTN9-2 onto a dithiobis-succinimidyl propionate (DSP) self-assembled monolayer on a gold electrode. The resulting immunosensor effectively captures <i>Bradyrhizobium</i> sp. SUTN9-2 cells are present in samples, leading to changes in electrode impedance. The immunosensor demonstrated excellent specificity, a wide linear detection range of 1 × 10<sup>3</sup> to 1 × 10<sup>7</sup> CFU/mL (<i>R</i> <sup>2</sup> = 0.992), and a limit of detection approaching the single-cell detection limit. The compatibility of the sensor with a soil sample was also demonstrated. The platform holds great promise for the portable monitoring of soil biofertilizer levels and could also be adapted to other strains of interest.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-04T05:07:49.319Z","creation":"2026-05-05T03:12:33.903Z"},"accession":"S-EPMC12489705","cross_references":{"pubmed":["41048798"],"doi":["10.1021/acsomega.5c06583"]}}