{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(10)"],"submitter":["Du B"],"pubmed_abstract":["Recent advances in twistronics have revealed tunable optoelectronic properties in twisted bilayer graphene (tBLG), including angle-dependent dielectric responses and enhanced light absorption due to van Hove singularity (VHS). However, achieving high photoresponsivity in tBLG-based sensors typically requires intense illumination. We present an ultrasensitive optoelectronic biosensor integrating tBLG superlattices with Au nanodisks and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a via DNA origami. By aligning the 9.4° tBLG's VHS absorption spectrum with Au nanodisks' plasmonic resonance at 60 μW, we achieve a 7-fold photocurrent enhancement over pristine tBLG. CRISPR-Cas12a-mediated trans-cleavage dynamically modulates the local dielectric environment, enabling sub-femtomolar (44.63 attomolar, aM) nucleic acid detection without external amplification. Clinical validation using lung cancer samples shows high concordance with quantitative polymerase chain reaction (qPCR), demonstrating real-time, label-free detection of microRNA (miRNA). This hybrid platform combines moiré-engineered optoelectronics with programmable bio-nanoarrays, offering a scalable solution for precision diagnostics with ultralow detection limits and rapid response times."],"journal":["National science review"],"pagination":["nwaf357"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12492000"],"repository":["biostudies-literature"],"pubmed_title":["Ultrasensitive optoelectronic biosensor arrays based on twisted bilayer graphene superlattice."],"pmcid":["PMC12492000"],"pubmed_authors":["Liu Z","Du B","Chen Z","Tian X","Ge Y","Wei S","Zhang H","Gao H","Chen C","Chen S","Tung J","Fixler D"],"additional_accession":[]},"is_claimable":false,"name":"Ultrasensitive optoelectronic biosensor arrays based on twisted bilayer graphene superlattice.","description":"Recent advances in twistronics have revealed tunable optoelectronic properties in twisted bilayer graphene (tBLG), including angle-dependent dielectric responses and enhanced light absorption due to van Hove singularity (VHS). However, achieving high photoresponsivity in tBLG-based sensors typically requires intense illumination. We present an ultrasensitive optoelectronic biosensor integrating tBLG superlattices with Au nanodisks and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a via DNA origami. By aligning the 9.4° tBLG's VHS absorption spectrum with Au nanodisks' plasmonic resonance at 60 μW, we achieve a 7-fold photocurrent enhancement over pristine tBLG. CRISPR-Cas12a-mediated trans-cleavage dynamically modulates the local dielectric environment, enabling sub-femtomolar (44.63 attomolar, aM) nucleic acid detection without external amplification. Clinical validation using lung cancer samples shows high concordance with quantitative polymerase chain reaction (qPCR), demonstrating real-time, label-free detection of microRNA (miRNA). This hybrid platform combines moiré-engineered optoelectronics with programmable bio-nanoarrays, offering a scalable solution for precision diagnostics with ultralow detection limits and rapid response times.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-05-31T03:08:23.902Z","creation":"2026-05-31T03:06:51.205Z"},"accession":"S-EPMC12492000","cross_references":{"pubmed":["41049410"],"doi":["10.1093/nsr/nwaf357"]}}