biostudies-literatureUnknown36(10)Macturk ELCamille and Henry Dreyfus FoundationRestek Academic Support ProgramFingerprints are routinely used as evidence in forensic investigations. Fingermarks, any mark left by a donor whether a complete print or not, include sweat and oil excreted by the donor. The chemical components of fingermarks are typically analyzed by gas chromatography-mass spectrometry (GC-MS). Complexity from the number of endogenous and exogenous components associated with fingermarks tends to cause challenging coelutions in resulting chromatograms. In these scenarios, nontargeted analysis can provide substantial benefits over traditional targeted methods that exist in the literature. In this proof-of-concept study, a nontargeted method for analyzing fingermarks was developed and optimized using comprehensive two-dimensional gas chromatography (GC×GC-TOFMS). Two different methods for Journal of the American Society for Mass Spectrometry2299-2309https://www.ebi.ac.uk/biostudies/studies/S-EPMC12492389biostudies-literatureNontargeted Screening of Fingermark Residue Using Comprehensive Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry for Future Use in Forensic Applications.PMC12492389Perrault Uptmor KAMacturk ELfalseNontargeted Screening of Fingermark Residue Using Comprehensive Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry for Future Use in Forensic Applications.Fingerprints are routinely used as evidence in forensic investigations. Fingermarks, any mark left by a donor whether a complete print or not, include sweat and oil excreted by the donor. The chemical components of fingermarks are typically analyzed by gas chromatography-mass spectrometry (GC-MS). Complexity from the number of endogenous and exogenous components associated with fingermarks tends to cause challenging coelutions in resulting chromatograms. In these scenarios, nontargeted analysis can provide substantial benefits over traditional targeted methods that exist in the literature. In this proof-of-concept study, a nontargeted method for analyzing fingermarks was developed and optimized using comprehensive two-dimensional gas chromatography (GC×GC-TOFMS). Two different methods for 2025-01-01T00:00:00Z2025 Oct2026-06-04T02:32:03.499Z2026-05-04T03:13:56.473ZS-EPMC124923894092491210.1021/jasms.5c00258