{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(1)"],"submitter":["Huang M"],"pubmed_abstract":["The purpose of this research is to examine the impact of key type VI secretion system (T6SS) proteins hemolysin coregulated protein (Hcp) and valine-glycine repeat protein G (VgrG) on the metabolism of Acinetobacter baumannii (A. baumannii). Homologous recombination technology was used to construct hcp knockout strain (ATCC17978Δhcp), vgrG knockout strain (ATCC17978ΔvgrG), and a combined hcp and vgrG knockout strain (ATCC17978ΔhcpΔvgrG), with the wild-type A. baumannii strain (ATCC17978) used as a control. These strains were co-cultured with human pulmonary alveolar epithelial cells (HPAEpiC), respectively. Subsequently, a non-targeted metabolomic analysis of the co-culture supernatant, bacteria, and cells was conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In th"],"journal":["Scientific reports"],"pagination":["34632"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12494896"],"repository":["biostudies-literature"],"pubmed_title":["Impact of hcp and vgrG on Acinetobacter baumannii biofilm formation during infection of human pulmonary alveolar epithelial cells."],"pmcid":["PMC12494896"],"pubmed_authors":["Qin P","Yang W","Liu M","Zhang Y","Huang M","Yu D"],"additional_accession":[]},"is_claimable":false,"name":"Impact of hcp and vgrG on Acinetobacter baumannii biofilm formation during infection of human pulmonary alveolar epithelial cells.","description":"The purpose of this research is to examine the impact of key type VI secretion system (T6SS) proteins hemolysin coregulated protein (Hcp) and valine-glycine repeat protein G (VgrG) on the metabolism of Acinetobacter baumannii (A. baumannii). Homologous recombination technology was used to construct hcp knockout strain (ATCC17978Δhcp), vgrG knockout strain (ATCC17978ΔvgrG), and a combined hcp and vgrG knockout strain (ATCC17978ΔhcpΔvgrG), with the wild-type A. baumannii strain (ATCC17978) used as a control. These strains were co-cultured with human pulmonary alveolar epithelial cells (HPAEpiC), respectively. Subsequently, a non-targeted metabolomic analysis of the co-culture supernatant, bacteria, and cells was conducted using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In th","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-04T02:04:26.008Z","creation":"2026-05-04T03:13:44.437Z"},"accession":"S-EPMC12494896","cross_references":{"pubmed":["41044369"],"doi":["10.1038/s41598-025-18287-1"]}}