biostudies-literatureUnknown69He Q<h4>Background</h4>The poor prognosis of metastatic prostate cancer (PCa) poses a major burden on both patients and the healthcare system. FAM111 trypsin-like peptidase B (FAM111B) is related to the development and progression of a wide array of cancers, but its role in PCa remains poorly understood.<h4>Methods</h4>Primary cells were extracted from subcutaneous and pulmonary metastatic tumors and were used to verify differences in metastatic potential through wound healing assay, Transwell assay, soft agar colony formation assay, and in vivo pulmonary metastasis reformation assays. The key differentially expressed gene FAM111B related to metastatic prostate cancer (mPCa) was identified through transcriptomic combination analysis, proteomic analysis, quantitative real-time fluorescent polymNeoplasia (New York, N.Y.)101227https://www.ebi.ac.uk/biostudies/studies/S-EPMC12495446biostudies-literatureFAM111B enhances glycolysis and promotes metastasis of prostate cancer by upregulating LDHA.PMC12495446Li HChen KChen JWang CXing YJu YChen LHe QZhang PLi XLv FCheng LCong YYu ZSong YfalseFAM111B enhances glycolysis and promotes metastasis of prostate cancer by upregulating LDHA.<h4>Background</h4>The poor prognosis of metastatic prostate cancer (PCa) poses a major burden on both patients and the healthcare system. FAM111 trypsin-like peptidase B (FAM111B) is related to the development and progression of a wide array of cancers, but its role in PCa remains poorly understood.<h4>Methods</h4>Primary cells were extracted from subcutaneous and pulmonary metastatic tumors and were used to verify differences in metastatic potential through wound healing assay, Transwell assay, soft agar colony formation assay, and in vivo pulmonary metastasis reformation assays. The key differentially expressed gene FAM111B related to metastatic prostate cancer (mPCa) was identified through transcriptomic combination analysis, proteomic analysis, quantitative real-time fluorescent polym2025-01-01T00:00:00Z2025 Nov2026-07-15T05:47:03.771Z2026-06-29T03:19:41.136ZS-EPMC124954464097597710.1016/j.neo.2025.101227