{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15"],"submitter":["Fang J"],"pubmed_abstract":["<h4>Objective</h4>This study aimed to explore the characteristics intratumoral microbiome in oral squamous cell carcinoma, and elucidate the interplay between intratumoral microbial profiles (relative abundance/absolute load) and tumor-infiltrating lymphocytes markers (CD4<sup>+</sup>/CD8<sup>+</sup>/FOXP3) and PD-L1 in oral squamous cell carcinoma.<h4>Methods</h4>We analyzed 45 OSCC tissue samples alongside paired paracancerous (n=10) and normal oral microbiota controls (n=8). Microbial composition was characterized by 16S rRNA sequencing (V3-V4 regions), with bacterial load quantified via qPCR targeting the V4-1 region. Tumor-infiltrating lymphocytes markers were assessed by immunohistochemistry.<h4>Results</h4><i>Spirochaetota</i> was concentrated in the CD4<sup>+</sup> as well as CD8<s"],"journal":["Frontiers in oncology"],"pagination":["1616928"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12497615"],"repository":["biostudies-literature"],"pubmed_title":["Intratumoral microbial abundance and load influence the immune microenvironment of oral squamous cell carcinoma."],"pmcid":["PMC12497615"],"pubmed_authors":["Wu M","Fang J","Zhang T","Shen H","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"Intratumoral microbial abundance and load influence the immune microenvironment of oral squamous cell carcinoma.","description":"<h4>Objective</h4>This study aimed to explore the characteristics intratumoral microbiome in oral squamous cell carcinoma, and elucidate the interplay between intratumoral microbial profiles (relative abundance/absolute load) and tumor-infiltrating lymphocytes markers (CD4<sup>+</sup>/CD8<sup>+</sup>/FOXP3) and PD-L1 in oral squamous cell carcinoma.<h4>Methods</h4>We analyzed 45 OSCC tissue samples alongside paired paracancerous (n=10) and normal oral microbiota controls (n=8). Microbial composition was characterized by 16S rRNA sequencing (V3-V4 regions), with bacterial load quantified via qPCR targeting the V4-1 region. Tumor-infiltrating lymphocytes markers were assessed by immunohistochemistry.<h4>Results</h4><i>Spirochaetota</i> was concentrated in the CD4<sup>+</sup> as well as CD8<s","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-05-06T03:16:21.815Z","creation":"2026-05-06T03:12:23.851Z"},"accession":"S-EPMC12497615","cross_references":{"pubmed":["41059290"],"doi":["10.3389/fonc.2025.1616928"]}}