biostudies-literatureUnknown15Xiao W<h4>Aims</h4>Spinal tuberculosis (STB) is a common form of extrapulmonary tuberculosis (ETB). However, the molecular mechanism of pathological injury in STB remains unclear. The purpose of this study was to explore the pathogenic mechanism of STB, and compare it with <i>Escherichia coli</i> (E.coli) bone infections and lumbar degenerative disease (LDD) patients.<h4>Main methods</h4>In this study, the infected lumbar spine bone tissue of STB patients was collected for the infection group. LDD patients and E.coli lumbar spine infection (SEcoli) patients were collected for the non-M.TB infection group. Proteins from the bone tissue were extracted for 4D-Label Free Proteomics (4D-LFQ) analysis to compare the pathogenesis and immune mechanisms of STB and SEcoli.<h4>Key findings</h4>The osteoclaFrontiers in cellular and infection microbiology1629805https://www.ebi.ac.uk/biostudies/studies/S-EPMC12497785biostudies-literatureMolecular mechanisms related to bone damage in spinal tuberculosis revealed by 4D-label-free proteomics analysis.PMC12497785Yang GGu YHuang WXiao WXu SSong SZhou TZhang LTang YfalseMolecular mechanisms related to bone damage in spinal tuberculosis revealed by 4D-label-free proteomics analysis.<h4>Aims</h4>Spinal tuberculosis (STB) is a common form of extrapulmonary tuberculosis (ETB). However, the molecular mechanism of pathological injury in STB remains unclear. The purpose of this study was to explore the pathogenic mechanism of STB, and compare it with <i>Escherichia coli</i> (E.coli) bone infections and lumbar degenerative disease (LDD) patients.<h4>Main methods</h4>In this study, the infected lumbar spine bone tissue of STB patients was collected for the infection group. LDD patients and E.coli lumbar spine infection (SEcoli) patients were collected for the non-M.TB infection group. Proteins from the bone tissue were extracted for 4D-Label Free Proteomics (4D-LFQ) analysis to compare the pathogenesis and immune mechanisms of STB and SEcoli.<h4>Key findings</h4>The osteocla2025-01-01T00:00:00Z20252026-06-04T06:56:15.52Z2026-05-06T03:13:08.414ZS-EPMC124977854105904110.3389/fcimb.2025.1629805