biostudies-literatureFu YXNational Natural Science Foundation of China34654https://www.ebi.ac.uk/biostudies/studies/S-EPMC12497871biostudies-literatureUnknown15(1)Lysosomal autophagy plays a critical role in ensuring the continuity of autophagic flux, however bioinformatics studies investigating biomarkers associated with lysosomal autophagy in cerebral ischemia-reperfusion injury(CIRI) remain scarce. Three datasets (GSE61616, GSE97537, and GSE82146) were retrieved from the GEO database. After batch correction, GSE61616 and GSE97537 were integrated into a combined dataset, while GSE82146 served as the validation set. Lysosomal autophagy-related genes (LRGs) obtained from the GeneCards database were intersected with differentially expressed genes (DEGs) identified between the CIRI group and the control group, resulting in the identification of 36 lysosomal autophagy-related differentially expressed genes (LRDEGs). Functional enrichment analysis usingScientific reportsVamp8 modulates cerebral ischemia-reperfusion injury via the autophagy-lysosome pathway.PMC124978718230153882071351Fu YXYin XSu TCui LCao JLang YfalseVamp8 modulates cerebral ischemia-reperfusion injury via the autophagy-lysosome pathway.Lysosomal autophagy plays a critical role in ensuring the continuity of autophagic flux, however bioinformatics studies investigating biomarkers associated with lysosomal autophagy in cerebral ischemia-reperfusion injury(CIRI) remain scarce. Three datasets (GSE61616, GSE97537, and GSE82146) were retrieved from the GEO database. After batch correction, GSE61616 and GSE97537 were integrated into a combined dataset, while GSE82146 served as the validation set. Lysosomal autophagy-related genes (LRGs) obtained from the GeneCards database were intersected with differentially expressed genes (DEGs) identified between the CIRI group and the control group, resulting in the identification of 36 lysosomal autophagy-related differentially expressed genes (LRDEGs). Functional enrichment analysis using2025-01-01T00:00:00Z2025 Oct2026-06-04T04:48:17.066Z2026-05-05T03:12:36.034ZS-EPMC124978714104737610.1038/s41598-025-20879-w