biostudies-literatureUnknown13Ploypetch S<h4>Background</h4>Direct reprogramming of somatic cells into induced neural stem cells (iNSCs) holds strong potential for regenerative medicine, especially in large animal models like pigs, which are crucial for translational and preclinical research. However, the molecular mechanisms underlying porcine fibroblast-to-iNSC reprogramming and subsequent differentiation remain poorly understood at the proteomic level.<h4>Methods</h4>To map the proteomic landscapes associated with reprogramming and differentiation, we performed unbiased label-free discovery proteomics (nano-LC-MS/MS) and targeted SWATH-MS quantification. Proteomes of porcine tail fibroblasts (PTFs; passage 3), two porcine iNSC lines (piNSCs; VSMUi002-B and VSMUi002-E, passage 20), and their differentiated progeny (piNSCs-NGs; PeerJe20120https://www.ebi.ac.uk/biostudies/studies/S-EPMC12499565biostudies-literatureProteomic landscape of porcine induced neural stem cell reprogramming and differentiation.PMC12499565Na Nakorn PPloypetch SMuangthong TPannengpetch SSakcamduang WChaisilp NChaiwattanarungruengpaisan SPhochantachinda SRungarunlert SChakritbudsabong WfalseProteomic landscape of porcine induced neural stem cell reprogramming and differentiation.<h4>Background</h4>Direct reprogramming of somatic cells into induced neural stem cells (iNSCs) holds strong potential for regenerative medicine, especially in large animal models like pigs, which are crucial for translational and preclinical research. However, the molecular mechanisms underlying porcine fibroblast-to-iNSC reprogramming and subsequent differentiation remain poorly understood at the proteomic level.<h4>Methods</h4>To map the proteomic landscapes associated with reprogramming and differentiation, we performed unbiased label-free discovery proteomics (nano-LC-MS/MS) and targeted SWATH-MS quantification. Proteomes of porcine tail fibroblasts (PTFs; passage 3), two porcine iNSC lines (piNSCs; VSMUi002-B and VSMUi002-E, passage 20), and their differentiated progeny (piNSCs-NGs; 2025-01-01T00:00:00Z20252026-05-06T03:19:16.205Z2026-05-06T03:13:07.454ZS-EPMC124995654105940810.7717/peerj.20120