{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ju CW"],"funding":["NHGRI NIH HHS"],"pagination":["8863"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12501074"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Dihydrouridine (D) is an abundant RNA modification, yet its roles in mammals remain poorly understood due to limited detection methods. We even do not have a comprehensive profile of D site location and modification stoichiometry in tRNA. Here, we introduce Chemical Reduction Assisted Cytosine Incorporation sequencing (CRACI), a highly sensitive, quantitative approach for mapping D at single-base resolution. Using CRACI, we generate the transcriptome-wide maps of D in both cytoplasmic and mitochondrial tRNAs from mammals and plants. We uncover D sites in mitochondrial tRNAs and identify DUS2L as the 'writer' protein responsible for human mitochondrial tRNAs. Furthermore, we demonstrate that most D modifications have a limited impact on tRNA stability, except for D20a, which also exhibits c"],"journal":["Nature communications"],"pubmed_title":["Quantitative CRACI reveals transcriptome-wide distribution of RNA dihydrouridine at base resolution."],"pmcid":["PMC12501074"],"funding_grant_id":["RM1 HG008935"],"pubmed_authors":["Ju CW","Li H","Zou J","He C","Cui L","Ye C","Xia P","Han Z","Zhong Y","Liu S","Shah H","Yang F","Zhang LS","Liu Y","Ge R","Jiang B","Zhu X","Shen S","Liu B","Ji Y","Wei J","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"Quantitative CRACI reveals transcriptome-wide distribution of RNA dihydrouridine at base resolution.","description":"Dihydrouridine (D) is an abundant RNA modification, yet its roles in mammals remain poorly understood due to limited detection methods. We even do not have a comprehensive profile of D site location and modification stoichiometry in tRNA. Here, we introduce Chemical Reduction Assisted Cytosine Incorporation sequencing (CRACI), a highly sensitive, quantitative approach for mapping D at single-base resolution. Using CRACI, we generate the transcriptome-wide maps of D in both cytoplasmic and mitochondrial tRNAs from mammals and plants. We uncover D sites in mitochondrial tRNAs and identify DUS2L as the 'writer' protein responsible for human mitochondrial tRNAs. Furthermore, we demonstrate that most D modifications have a limited impact on tRNA stability, except for D20a, which also exhibits c","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-04T05:35:58.278Z","creation":"2026-05-05T03:13:01.54Z"},"accession":"S-EPMC12501074","cross_references":{"pubmed":["41053065"],"doi":["10.1038/s41467-025-63918-w"]}}