biostudies-literatureMaranta NMinistero dell'Università e della RicercaLinnea SA74https://www.ebi.ac.uk/biostudies/studies/S-EPMC12502145biostudies-literatureUnknown7(1)<h4>Background</h4>The use of Cannabis sativa L. (Cannabis) was reported by observational studies on inflammatory bowel diseases (IBD) patients. However, this indication is poorly supported by clinical trials. Several pre-clinical studies demonstrated the anti-inflammatory activity of Δ⁹-tetrahydrocannabinol (Δ⁹-THC) and cannabidiol (CBD) at intestinal level. On the contrary, minor cannabinoids, such as cannabigerol (CBG), were less investigated. Moreover, several authors suggested that complex Cannabis extracts might display a higher efficacy in respect to pure cannabinoids against inflammatory disorders.<h4>Methods</h4>This study was aimed at investigating the role of Cannabis extracts, standardized in CBD and CBG content, in a model of in vitro-induced intestinal inflammation using CaCoJournal of cannabis researchEfficacy of non-psychotropic Cannabis sativa L. standardized extracts in a model of intestinal inflammation.PMC12502145not applicableCiriello USangiovanni EMartinelli GFumagalli MDell'Agli MRossini NPozzoli CMaranta NSonzogni EPiazza SPaladino GfalseEfficacy of non-psychotropic Cannabis sativa L. standardized extracts in a model of intestinal inflammation.<h4>Background</h4>The use of Cannabis sativa L. (Cannabis) was reported by observational studies on inflammatory bowel diseases (IBD) patients. However, this indication is poorly supported by clinical trials. Several pre-clinical studies demonstrated the anti-inflammatory activity of Δ⁹-tetrahydrocannabinol (Δ⁹-THC) and cannabidiol (CBD) at intestinal level. On the contrary, minor cannabinoids, such as cannabigerol (CBG), were less investigated. Moreover, several authors suggested that complex Cannabis extracts might display a higher efficacy in respect to pure cannabinoids against inflammatory disorders.<h4>Methods</h4>This study was aimed at investigating the role of Cannabis extracts, standardized in CBD and CBG content, in a model of in vitro-induced intestinal inflammation using CaCo2025-01-01T00:00:00Z2025 Oct2026-06-04T06:30:21.099Z2026-06-01T03:06:45.961ZS-EPMC125021454105384310.1186/s42238-025-00335-2