biostudies-literatureUnknownHymoller KM<h4>Introduction</h4>The complement system plays a crucial role in bridging innate and adaptive immune responses. When activated, a proteolytic cascade leads to pathogen destruction. It is initiated via the recognition of foreign structures by three pathways: the classical, the lectin, and the alternative. This study focuses on the lectin pathway and the role of four pattern recognition molecules (PRMs), mannan-binding lectin, H-ficolin, L-ficolin, and M-ficolin, in the recognition of microbial patterns and the initiation of complement activation. These PRMs bind to specific carbohydrate structures; each PRM has unique ligand specificities. We investigated the PRM interactions with lipopolysaccharide (LPS) of Gram-negative bacteria.<h4>Methods</h4>Utilizing a microarray of 120 distinct LPSJournal of innate immunity1-27https://www.ebi.ac.uk/biostudies/studies/S-EPMC12503535biostudies-literatureDifferential Recognition of Lipopolysaccharide O-Antigens by the Pattern Recognition Molecules MBL and Ficolins of the Complement System.PMC12503535Thiel SCrone LHymoller KMHennet TfalseDifferential Recognition of Lipopolysaccharide O-Antigens by the Pattern Recognition Molecules MBL and Ficolins of the Complement System.<h4>Introduction</h4>The complement system plays a crucial role in bridging innate and adaptive immune responses. When activated, a proteolytic cascade leads to pathogen destruction. It is initiated via the recognition of foreign structures by three pathways: the classical, the lectin, and the alternative. This study focuses on the lectin pathway and the role of four pattern recognition molecules (PRMs), mannan-binding lectin, H-ficolin, L-ficolin, and M-ficolin, in the recognition of microbial patterns and the initiation of complement activation. These PRMs bind to specific carbohydrate structures; each PRM has unique ligand specificities. We investigated the PRM interactions with lipopolysaccharide (LPS) of Gram-negative bacteria.<h4>Methods</h4>Utilizing a microarray of 120 distinct LPS2025-01-01T00:00:00Z2025 Jul2026-06-04T06:18:25.4Z2026-05-06T03:12:24.954ZS-EPMC125035354066418210.1159/000547441