{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["24(10)"],"submitter":["Hou C"],"pubmed_abstract":["O-linked β-N-acetylglucosamine (O-GlcNAc) modification (i.e., O-GlcNAcylation) on proteins is an essential modification in physiology and pathology. Although O-GlcNAcylation is functionally critical, its analysis has been challenging. Despite the existence of a number of methods developed in the past years, which one(s) might have the best performance is largely unclear. To that end, we conducted a rigorous comparison of several cleavable bioorthogonal biotin-alkyne probes which showed promise for sensitive O-GlcNAc proteomics. In brief, we developed chemoenzymatic labeling/click chemistry-based analytical workflows for O-GlcNAc proteomics by utilizing four cleavable bioorthogonal probes, including photocleavabe-biotin-alkyne (PC-biotin-alkyne), dialkoxydiphenylsilane-biotin-alkyne (DADPS-"],"journal":["Molecular & cellular proteomics : MCP"],"pagination":["101064"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12506516"],"repository":["biostudies-literature"],"pubmed_title":["Comprehensive Evaluation of Cleavable Bioorthogonal Probes for Site-Specific O-GlcNAc Proteomics."],"pmcid":["PMC12506516"],"pubmed_authors":["Pak DTS","Zhang H","Hart GW","Deng J","Byers S","Pei H","Ma J","Hou C","Wang X","Moremen KW","Levi M"],"additional_accession":[]},"is_claimable":false,"name":"Comprehensive Evaluation of Cleavable Bioorthogonal Probes for Site-Specific O-GlcNAc Proteomics.","description":"O-linked β-N-acetylglucosamine (O-GlcNAc) modification (i.e., O-GlcNAcylation) on proteins is an essential modification in physiology and pathology. Although O-GlcNAcylation is functionally critical, its analysis has been challenging. Despite the existence of a number of methods developed in the past years, which one(s) might have the best performance is largely unclear. To that end, we conducted a rigorous comparison of several cleavable bioorthogonal biotin-alkyne probes which showed promise for sensitive O-GlcNAc proteomics. In brief, we developed chemoenzymatic labeling/click chemistry-based analytical workflows for O-GlcNAc proteomics by utilizing four cleavable bioorthogonal probes, including photocleavabe-biotin-alkyne (PC-biotin-alkyne), dialkoxydiphenylsilane-biotin-alkyne (DADPS-","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Aug","modification":"2026-06-04T09:01:06.437Z","creation":"2026-06-01T03:06:31.032Z"},"accession":"S-EPMC12506516","cross_references":{"pubmed":["40885482"],"doi":["10.1016/j.mcpro.2025.101064"]}}