{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["5(10)"],"submitter":["Ng HK"],"pubmed_abstract":["Understanding a protein's interactome provides crucial insight into its function and its contribution to disease. Traditional methods such as co-immunoprecipitations often fail to capture interactions that are dependent on native cellular architecture such as those found on the cellular membrane. While enzyme-based proximity labeling utilizing peroxidases or biotin ligases can achieve in situ interactome mapping, these approaches are limited by labeling radii, cellular engineering, and amino acid labeling biases. Antibody-guided µMap photoproximity labeling addresses the constraints of these alternative platforms. Here, we apply µMap photoproximity labeling to study the interactome of HER2 by using antibody-guided proximity labeling to target the endogenous protein, ablating the need for c"],"journal":["Current protocols"],"pagination":["e70216"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12507076"],"repository":["biostudies-literature"],"pubmed_title":["µMap Photoproximity Labeling on the Cell Surface."],"pmcid":["PMC12507076"],"pubmed_authors":["Ng HK","Seath CP","Douglas CJ"],"additional_accession":[]},"is_claimable":false,"name":"µMap Photoproximity Labeling on the Cell Surface.","description":"Understanding a protein's interactome provides crucial insight into its function and its contribution to disease. Traditional methods such as co-immunoprecipitations often fail to capture interactions that are dependent on native cellular architecture such as those found on the cellular membrane. While enzyme-based proximity labeling utilizing peroxidases or biotin ligases can achieve in situ interactome mapping, these approaches are limited by labeling radii, cellular engineering, and amino acid labeling biases. Antibody-guided µMap photoproximity labeling addresses the constraints of these alternative platforms. Here, we apply µMap photoproximity labeling to study the interactome of HER2 by using antibody-guided proximity labeling to target the endogenous protein, ablating the need for c","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-05-07T03:18:41.664Z","creation":"2026-05-07T03:12:22.258Z"},"accession":"S-EPMC12507076","cross_references":{"pubmed":["41060711"],"doi":["10.1002/cpz1.70216"]}}