biostudies-literatureFarfan-Garcia AEMinisterio de Ciencia, TecnologÃa e Innovacióne0334029https://www.ebi.ac.uk/biostudies/studies/S-EPMC12507217biostudies-literatureUnknown20(10)Antimicrobial resistance represents a critical global public health challenge, leading to increased mortality and morbidity due to the ineffectiveness of current antibiotics against bacterial infections. Antimicrobial peptides (AMPs) offer a promising alternative for treating bacterial infections because of their broad-spectrum activity, biocompatibility, and rapid bactericidal action. Recent studies have demonstrated that Ib-M peptides exhibit bactericidal activity against pathogenic Escherichia coli clinical isolates. The objective of this study was to evaluate the mechanisms by which Ib-M peptides destabilize and disrupts E. coli membranes. We showed by dilutions assays that Ib-M peptides had a minimum inhibitory concentration (MIC) of 12.5 µM against E. coli. Electron microscopy studiePloS oneStructural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides.PMC12507217129980763392 CT-760-2018Farfan-Garcia AEGomez-Duarte OGRincon-Baron EJHernandez-Penaranda IPFlorez-Castillo JMTorres-Rodriguez GAfalseStructural and functional analysis of Escherichia coli membrane disruption by Ib-M peptides.Antimicrobial resistance represents a critical global public health challenge, leading to increased mortality and morbidity due to the ineffectiveness of current antibiotics against bacterial infections. Antimicrobial peptides (AMPs) offer a promising alternative for treating bacterial infections because of their broad-spectrum activity, biocompatibility, and rapid bactericidal action. Recent studies have demonstrated that Ib-M peptides exhibit bactericidal activity against pathogenic Escherichia coli clinical isolates. The objective of this study was to evaluate the mechanisms by which Ib-M peptides destabilize and disrupts E. coli membranes. We showed by dilutions assays that Ib-M peptides had a minimum inhibitory concentration (MIC) of 12.5 µM against E. coli. Electron microscopy studie2025-01-01T00:00:00Z20252026-06-04T08:47:28.492Z2026-05-07T03:12:16.956ZS-EPMC125072174106101610.1371/journal.pone.0334029