{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Miao J"],"funding":["Eunice Kennedy Shriver National Institute of Child Health & Human Development","Michigan State University","NICHD","National Science Foundation"],"pagination":["gkaf957"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12507516"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(18)"],"pubmed_abstract":["Repressing transposable elements via piRNAs represents a critical defense mechanism for germ cells to maintain genomic integrity. The primary piRNA biogenesis largely occurs at intermitochondrial cement (IMC), which is characterized by uniquely clustered mitochondria and ribonucleoproteins as \"cementing material.\" RNA-binding proteins at IMC, such as MILI, are essential for piRNA biogenesis. However, MILI proteins do not possess mitochondrial localization signals; thus, they must rely on other proteins to functionally communicate with IMC. In this study, we identified GASZ as a crucial interacting partner for MILI at IMC from prospermatogonia to spermatocytes. We found that GASZ proteins at mitochondria directly recruited MILI to IMC for piRNA biogenesis. Abolishing GASZ-MILI interaction i"],"journal":["Nucleic acids research"],"pubmed_title":["GASZ directly recruits MILI to the intermitochondrial cement for piRNA biogenesis and male germ cell development."],"pmcid":["PMC12507516"],"funding_grant_id":["2042908","HD110627","T32HD087166"],"pubmed_authors":["Gong D","Marshall M","Zhang Z","Xie H","Zhang J","Wang C","Nguyen D","Wang H","Wang Y","Miao J","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"GASZ directly recruits MILI to the intermitochondrial cement for piRNA biogenesis and male germ cell development.","description":"Repressing transposable elements via piRNAs represents a critical defense mechanism for germ cells to maintain genomic integrity. The primary piRNA biogenesis largely occurs at intermitochondrial cement (IMC), which is characterized by uniquely clustered mitochondria and ribonucleoproteins as \"cementing material.\" RNA-binding proteins at IMC, such as MILI, are essential for piRNA biogenesis. However, MILI proteins do not possess mitochondrial localization signals; thus, they must rely on other proteins to functionally communicate with IMC. In this study, we identified GASZ as a crucial interacting partner for MILI at IMC from prospermatogonia to spermatocytes. We found that GASZ proteins at mitochondria directly recruited MILI to IMC for piRNA biogenesis. Abolishing GASZ-MILI interaction i","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-07T03:22:25.878Z","creation":"2026-05-07T03:12:47.674Z"},"accession":"S-EPMC12507516","cross_references":{"pubmed":["41063345"],"doi":["10.1093/nar/gkaf957"]}}