biostudies-literatureUnknown57(9)Lee YGthe ministry of Science and ICT of the Korean GovernmentRet finger protein (RFP, also known as TRIM27) is a multifunctional E3 ubiquitin ligase implicated in transcriptional regulation. While previously reported to repress myogenesis, its role in adipose tissue metabolism remains unclear. Here, using both global and adipocyte-specific RFP-knockout male mice subjected to high-fat diet feeding, we found that RFP deficiency markedly attenuated body weight gain, adipose tissue expansion and adipocyte hypertrophy, while improving glucose tolerance, insulin sensitivity and circulating lipid profiles. Indirect calorimetry demonstrated significantly increased whole-body energy expenditure, independent of food intake or physical activity. Consistent with these murine findings, RFP expression was elevated in omental adipose tissue from obese human subjecExperimental & molecular medicine2052-2066https://www.ebi.ac.uk/biostudies/studies/S-EPMC12508092biostudies-literatureRet finger protein deficiency attenuates adipogenesis in male mice with high fat diet-induced obesity.PMC12508092Shin SKook HYu HCLee SJNam KILim YKim HSJoung HKwon DHLee YGPark BHPark SYEom GHJeong ACho HJfalseRet finger protein deficiency attenuates adipogenesis in male mice with high fat diet-induced obesity.Ret finger protein (RFP, also known as TRIM27) is a multifunctional E3 ubiquitin ligase implicated in transcriptional regulation. While previously reported to repress myogenesis, its role in adipose tissue metabolism remains unclear. Here, using both global and adipocyte-specific RFP-knockout male mice subjected to high-fat diet feeding, we found that RFP deficiency markedly attenuated body weight gain, adipose tissue expansion and adipocyte hypertrophy, while improving glucose tolerance, insulin sensitivity and circulating lipid profiles. Indirect calorimetry demonstrated significantly increased whole-body energy expenditure, independent of food intake or physical activity. Consistent with these murine findings, RFP expression was elevated in omental adipose tissue from obese human subjec2025-01-01T00:00:00Z2025 Sep2026-06-11T05:11:21.198Z2026-06-11T03:08:23.284ZS-EPMC125080924096814810.1038/s12276-025-01553-7