biostudies-literatureUnknown15(1)Abozaid DDental composites are widely used restorative materials but secondary caries to the restored tooth, polymerization shrinkage, and mechanical failure, limiting their longevity. Incorporating nanoparticles synthesized via eco-friendly methods offers a promising strategy to enhance composite performance. This study introduces a novel, eco-friendly strategy to enhance dental composite resins by incorporating TiO₂-NPs synthesized using Vitis vinifera extract. This study uniquely employs Vitis vinifera, rich in flavonoids and phenolics, as a reducing and capping agent, and for the first time integrates molecular docking analysis to explore the mechanistic antimicrobial interaction between TiO₂-NPs and Streptococcus mutans glucosyltransferase. The research evaluates the impact of these green-syntScientific reports35042https://www.ebi.ac.uk/biostudies/studies/S-EPMC12508154biostudies-literatureAntimicrobial mechanical and molecular docking analysis of dental composite resin incorporating green synthesized titanium dioxide nanoparticles from Vitis vinifera extract.PMC12508154El-Aal MAAbdelmonem MSaleh AMAzab AIbrahim MAEldin ZEAbozaid DfalseAntimicrobial mechanical and molecular docking analysis of dental composite resin incorporating green synthesized titanium dioxide nanoparticles from Vitis vinifera extract.Dental composites are widely used restorative materials but secondary caries to the restored tooth, polymerization shrinkage, and mechanical failure, limiting their longevity. Incorporating nanoparticles synthesized via eco-friendly methods offers a promising strategy to enhance composite performance. This study introduces a novel, eco-friendly strategy to enhance dental composite resins by incorporating TiO₂-NPs synthesized using Vitis vinifera extract. This study uniquely employs Vitis vinifera, rich in flavonoids and phenolics, as a reducing and capping agent, and for the first time integrates molecular docking analysis to explore the mechanistic antimicrobial interaction between TiO₂-NPs and Streptococcus mutans glucosyltransferase. The research evaluates the impact of these green-synt2025-01-01T00:00:00Z2025 Oct2026-06-04T07:42:50.274Z2026-05-06T03:12:58.877ZS-EPMC125081544106266910.1038/s41598-025-20989-5