{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pirozzi M"],"funding":["AIRC, Italy","Ministero dell'Università e della Ricerca (MUR)","Ministero dell'Università e della Ricerca"],"pagination":["4742-4765"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12508209"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(19)"],"pubmed_abstract":["The classical models of intra-Golgi transport envision a movement of cargoes from cis- to trans-Golgi, followed by their sorting at the trans-Golgi network (TGN). During this vectorial transport, the cargoes are processed by sequentially acting glycosylation enzymes. A number of studies challenged the vectorial transport model and proposed alternative transport routes bypassing either directional transport or the TGN. We have re-visited intra-Golgi transport using varied cargo synchronization protocols, employing both imaging and biochemical methods. We find that cargoes move vectorially across the Golgi stack and reach the TGN. Cell type-dependent variations in transport kinetics and the limited resolution of fluorescence microscopy could have influenced earlier discrepant interpretations"],"journal":["EMBO reports"],"pubmed_title":["Cargoes move from cis to trans-Golgi compartments and concentrate in the TGN before exiting."],"pmcid":["PMC12508209"],"funding_grant_id":["IG 15767","P2022MMPXH","FOE-EuroBioimaging","IG 20786","2022MZJR9X","P2022YA3LL","CN00000041 CN3 RNA"],"pubmed_authors":["Russo D","Quarto M","Luini A","Parashuraman S","Agliarulo I","Di Martino R","Beznoussenko GV","Mironov AA","Pirozzi M","Marzullo VM","Turacchio G"],"additional_accession":[]},"is_claimable":false,"name":"Cargoes move from cis to trans-Golgi compartments and concentrate in the TGN before exiting.","description":"The classical models of intra-Golgi transport envision a movement of cargoes from cis- to trans-Golgi, followed by their sorting at the trans-Golgi network (TGN). During this vectorial transport, the cargoes are processed by sequentially acting glycosylation enzymes. A number of studies challenged the vectorial transport model and proposed alternative transport routes bypassing either directional transport or the TGN. We have re-visited intra-Golgi transport using varied cargo synchronization protocols, employing both imaging and biochemical methods. We find that cargoes move vectorially across the Golgi stack and reach the TGN. Cell type-dependent variations in transport kinetics and the limited resolution of fluorescence microscopy could have influenced earlier discrepant interpretations","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-04T09:24:54.261Z","creation":"2026-05-07T03:13:04.43Z"},"accession":"S-EPMC12508209","cross_references":{"pubmed":["40903494"],"doi":["10.1038/s44319-025-00548-9"]}}