{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ummethum H"],"funding":["Deutsche Forschungsgemeinschaft (DFG)","Deutsche Forschungsgemeinschaft","European Research Council","Helmholtz Association"],"pagination":["4691-4722"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12508481"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(19)"],"pubmed_abstract":["The CGG triplet repeat binding protein 1 (CGGBP1) binds to CGG repeats and has several important cellular functions, but how this DNA sequence-specific binding factor affects transcription and replication processes is an open question. Here, we show that CGGBP1 binds human gene promoters containing short (< 5) CGG-repeat tracts prone to R-loop formation. Loss of CGGBP1 leads to deregulated transcription, transcription-replication-conflicts (TRCs) and accumulation of Serine-5 phosphorylated RNA polymerase II (RNAPII), indicative of promoter-proximal stalling and a defect in transcription elongation. Consistently, an episomal CGG-repeat-containing model locus as well as endogenous genes show deregulated transcription, R-loop accumulation and increased RNAPII chromatin occupancy in CGGBP1-dep"],"journal":["EMBO reports"],"pubmed_title":["The CGG triplet repeat binding protein 1 counteracts R-loop induced transcription-replication stress."],"pmcid":["PMC12508481"],"funding_grant_id":["852798","CRC1064,project ID 213249687"],"pubmed_authors":["Werner M","Erhard F","Chanou A","Weiβ M","Kruse E","Hauck SM","Konig AC","Trauner M","Hamperl S","Lalonde M","Murriello AC","Lee CSK","Ummethum H","Marquez-Gomez E","Ettinger A"],"additional_accession":[]},"is_claimable":false,"name":"The CGG triplet repeat binding protein 1 counteracts R-loop induced transcription-replication stress.","description":"The CGG triplet repeat binding protein 1 (CGGBP1) binds to CGG repeats and has several important cellular functions, but how this DNA sequence-specific binding factor affects transcription and replication processes is an open question. Here, we show that CGGBP1 binds human gene promoters containing short (< 5) CGG-repeat tracts prone to R-loop formation. Loss of CGGBP1 leads to deregulated transcription, transcription-replication-conflicts (TRCs) and accumulation of Serine-5 phosphorylated RNA polymerase II (RNAPII), indicative of promoter-proximal stalling and a defect in transcription elongation. Consistently, an episomal CGG-repeat-containing model locus as well as endogenous genes show deregulated transcription, R-loop accumulation and increased RNAPII chromatin occupancy in CGGBP1-dep","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-04T10:13:13.52Z","creation":"2026-05-08T03:10:30.651Z"},"accession":"S-EPMC12508481","cross_references":{"pubmed":["40859011"],"doi":["10.1038/s44319-025-00550-1"]}}