biostudies-literatureUnknown62Liu L<h4>Background</h4>M2-polarized tumor-associated macrophage infiltration is a key risk factor for poor prognosis in triple-negative breast cancer (TNBC). This study investigated the role of transcription elongation factor B polypeptide 2 (TCEB2) in regulating M2 macrophage polarization during TNBC development.<h4>Methods</h4>The expression of gene or protein was tested by qRT-PCR, western blot, and IHC. CCK8, colony formation, wound healing, and Transwell assays were used to evaluate TNBC cell malignant behaviors, including cell viability, proliferation, migration, and invasion. The secretion levels of cytokines were detected by ELISA. Ubiquitin-based IP assays were used to detect Slit2 ubiquitination. The combined relation between TCEB2, Slit2, and NEDD4 was investigated using Co-IP assayTranslational oncology102536https://www.ebi.ac.uk/biostudies/studies/S-EPMC12508586biostudies-literatureTCEB2 promotes M2 polarization of macrophages in triple negative breast cancer by mediating ubiquitination degradation of Slit2 through recruiting NEDD4.PMC12508586Yi JLiu LZeng JXiao WGong HfalseTCEB2 promotes M2 polarization of macrophages in triple negative breast cancer by mediating ubiquitination degradation of Slit2 through recruiting NEDD4.<h4>Background</h4>M2-polarized tumor-associated macrophage infiltration is a key risk factor for poor prognosis in triple-negative breast cancer (TNBC). This study investigated the role of transcription elongation factor B polypeptide 2 (TCEB2) in regulating M2 macrophage polarization during TNBC development.<h4>Methods</h4>The expression of gene or protein was tested by qRT-PCR, western blot, and IHC. CCK8, colony formation, wound healing, and Transwell assays were used to evaluate TNBC cell malignant behaviors, including cell viability, proliferation, migration, and invasion. The secretion levels of cytokines were detected by ELISA. Ubiquitin-based IP assays were used to detect Slit2 ubiquitination. The combined relation between TCEB2, Slit2, and NEDD4 was investigated using Co-IP assay2025-01-01T00:00:00Z2025 Sep2026-06-04T08:51:46.307Z2026-05-07T03:12:13.853ZS-EPMC125085864101488010.1016/j.tranon.2025.102536