{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hu H"],"funding":["National Natural Science Foundation of China","Natural Science Foundation of Jiangsu Province"],"pagination":["e70042"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12508693"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["58(10)"],"pubmed_abstract":["The E3 ubiquitin ligase RNF187, also known as RING domain AP1 coactivator-1, is a member of the RING finger family. RNF187 is indispensable for the proliferation and migration of GC-1 cells derived from mouse spermatogonia and GC-2 cells derived from spermatocytes. However, it remains unclear whether RNF187 plays a crucial role in the self-renewal and migration of human spermatogonial stem cells (SSCs). In this study, we observed a positive correlation between RNF187 expression and the proliferation and migration of human SSCs. Through co-immunoprecipitation and mass spectrometry analyses, we identified WD repeat-containing protein 77 (WDR77) as an interacting partner of RNF187. Specifically, RNF187 recognises the K118 site of WDR77 through lysine 48-linked polyubiquitination, subsequently"],"journal":["Cell proliferation"],"pubmed_title":["RNF187 Facilitates Proliferation and Migration of Human Spermatogonial Stem Cells Through WDR77 Polyubiquitination."],"pmcid":["PMC12508693"],"funding_grant_id":["BK20221376","32470899","82471641","82201762","82271633"],"pubmed_authors":["Xi X","Guo Y","Zhou T","Yu J","Wang K","Hu H","Huang X","Jiang B","Zheng B","Wu T","Chen X","Wu Y","Gao T"],"additional_accession":[]},"is_claimable":false,"name":"RNF187 Facilitates Proliferation and Migration of Human Spermatogonial Stem Cells Through WDR77 Polyubiquitination.","description":"The E3 ubiquitin ligase RNF187, also known as RING domain AP1 coactivator-1, is a member of the RING finger family. RNF187 is indispensable for the proliferation and migration of GC-1 cells derived from mouse spermatogonia and GC-2 cells derived from spermatocytes. However, it remains unclear whether RNF187 plays a crucial role in the self-renewal and migration of human spermatogonial stem cells (SSCs). In this study, we observed a positive correlation between RNF187 expression and the proliferation and migration of human SSCs. Through co-immunoprecipitation and mass spectrometry analyses, we identified WD repeat-containing protein 77 (WDR77) as an interacting partner of RNF187. Specifically, RNF187 recognises the K118 site of WDR77 through lysine 48-linked polyubiquitination, subsequently","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-04T08:54:47.749Z","creation":"2026-05-07T03:12:55.297Z"},"accession":"S-EPMC12508693","cross_references":{"pubmed":["40197797"],"doi":["10.1111/cpr.70042"]}}