{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["29"],"submitter":["Yu Y"],"pubmed_abstract":["Coccidiosis, which is primarily caused by Eimeria spp., poses a persistent challenge to poultry health and production worldwide. The emergence and spread of drug-resistant strains have significantly compromised the efficacy of anticoccidial therapies. We previously used RNA-seq to demonstrate that ribosomal protein L27 is differentially expressed in drug-sensitive and maduramicin-resistant strains of E. tenella (EtRPL27). In the present study, an RT-qPCR analysis showed that its expression is stage-specific, with the highest levels in sporozoites and second-generation merozoites. Immunofluorescence revealed both the cytoplasmic and partial surface localization of EtRPL27. Notably, EtRPL27 transcript levels were significantly elevated in a maduramicin-resistant strain relative to the drug-s"],"journal":["International journal for parasitology. Drugs and drug resistance"],"pagination":["100615"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12509730"],"repository":["biostudies-literature"],"pubmed_title":["Ribosomal protein L27 contributes to virulence and maduramicin resistance in Eimeria tenella."],"pmcid":["PMC12509730"],"pubmed_authors":["Huilin G","Guan G","Dong H","Zhao Q","Zhao F","Han H","Li L","Wang J","Yu Y","Zhu S"],"additional_accession":[]},"is_claimable":false,"name":"Ribosomal protein L27 contributes to virulence and maduramicin resistance in Eimeria tenella.","description":"Coccidiosis, which is primarily caused by Eimeria spp., poses a persistent challenge to poultry health and production worldwide. The emergence and spread of drug-resistant strains have significantly compromised the efficacy of anticoccidial therapies. We previously used RNA-seq to demonstrate that ribosomal protein L27 is differentially expressed in drug-sensitive and maduramicin-resistant strains of E. tenella (EtRPL27). In the present study, an RT-qPCR analysis showed that its expression is stage-specific, with the highest levels in sporozoites and second-generation merozoites. Immunofluorescence revealed both the cytoplasmic and partial surface localization of EtRPL27. Notably, EtRPL27 transcript levels were significantly elevated in a maduramicin-resistant strain relative to the drug-s","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-06-04T09:20:24.203Z","creation":"2026-05-07T03:12:55.31Z"},"accession":"S-EPMC12509730","cross_references":{"pubmed":["41004933"],"doi":["10.1016/j.ijpddr.2025.100615"]}}