{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["42(8-10)"],"submitter":["Oba Y"],"funding":["This study was supported by the Collaborative Project of the Departments of Science and Agriculture at Shizuoka University (to Yoko Kimura and Takashi Ushimaru), the Takeda Foundation (to Yoko Kimura), the Japan Society for the Promotion of Science (JSPS) (Grants-in-Aid for Scientific Research; grant No. 20K06620 and 23K05145 to Yoko Kimura, and 18K06212, 20H05317, and 21H02475 to Takashi Ushimaru), and the Institute for Fermentation, Osaka (grant No. G-2020-2-055 to Takashi Ushimaru)."],"pubmed_abstract":["The target of rapamycin complex 1 (TORC1) protein kinase plays an important role in regulating various cellular activities in response to nutrient availability. In this study, an autophagy-related protein 8 (atg8) mutant of Saccharomyces cerevisiae was highly sensitive to cellular processes in which TORC1 activity was inhibited by rapamycin treatment or by a mutated allele of KOG1 which encodes a subunit of TORC1. Atg8 exhibits both lipidation-dependent and -independent activities, each involving distinct factors. Lipidation of Atg8 is necessary for autophagy and functions with autophagy-related proteins like Atg7, whereas the lipidation-independent activities of Atg8 require Hfl1. The atg7Δhfl1Δ double mutant exhibited defects for the impaired TORC1 activities, suggesting that both lipida"],"journal":["Yeast (Chichester, England)"],"pagination":["161-168"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12511908"],"repository":["biostudies-literature"],"pubmed_title":["Cooperative Function of Atg8- and TORC1-Mediated Activities in Yeast."],"pmcid":["PMC12511908"],"pubmed_authors":["Oba Y","Katsuta H","Kimura Y","Higuchi M","Takahashi N","Koike N","Ushimaru T"],"additional_accession":[]},"is_claimable":false,"name":"Cooperative Function of Atg8- and TORC1-Mediated Activities in Yeast.","description":"The target of rapamycin complex 1 (TORC1) protein kinase plays an important role in regulating various cellular activities in response to nutrient availability. In this study, an autophagy-related protein 8 (atg8) mutant of Saccharomyces cerevisiae was highly sensitive to cellular processes in which TORC1 activity was inhibited by rapamycin treatment or by a mutated allele of KOG1 which encodes a subunit of TORC1. Atg8 exhibits both lipidation-dependent and -independent activities, each involving distinct factors. Lipidation of Atg8 is necessary for autophagy and functions with autophagy-related proteins like Atg7, whereas the lipidation-independent activities of Atg8 require Hfl1. The atg7Δhfl1Δ double mutant exhibited defects for the impaired TORC1 activities, suggesting that both lipida","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-07T03:22:20.746Z","creation":"2026-05-07T03:12:46.392Z"},"accession":"S-EPMC12511908","cross_references":{"pubmed":["40568955"],"doi":["10.1002/yea.4003"]}}